FastQCFastQC Report
Mon 25 Sept 2023
SRR387874.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387874.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19397736
Total Bases775.9 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAA4976382.565443719823798No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAA4061042.093563908695324No Hit
ATGTACACGGAGTCGACCCGCAACGTGAAAAAAAAAAAAA3245491.673128245481844No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAA2833751.4608663608990244No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAA2816511.4519787257646974No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAA2530921.304750203838221No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAA2158901.1129649357017748No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1428040.7361890067995565No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA1130710.5829082321771984No Hit
TGTTAATAGATAATATACGGATCTTAATAAAAAAAAAAAA1079620.5565701069444393No Hit
TTTGTTAATAGATAATATACGGATCTTAATAAAAAAAAAA1063700.5483629635953392No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA945240.48729398111202255No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAA883860.455651113098972No Hit
TTGTTAATAGATAATATACGGATCTTAATAAAAAAAAAAA867850.4473975725826973No Hit
AATACTGAATATCTAAATATTATTATTACTTAAAAAAAAA829910.4278385889982212No Hit
ATGTACACGGAGTCGACCCGCAACGCTAAAAAAAAAAAAA784080.40421212042477533No Hit
TTTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAA777820.4009849396857447No Hit
ATGTACACGGAGTCGACCCGCAACGCCAAAAAAAAAAAAA764430.39408207225832953No Hit
ATGTACACGGAGTCGACCCGCAACGCGGAAAAAAAAAAAA751120.38722044675729167No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAA689270.3553352824267739No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC680800.350968793471568No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA660060.3402768240582303No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAA635380.32755368977080623No Hit
TTTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAA628280.32389346880481307No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA538880.2778056160780825No Hit
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC523000.2696190936921711No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC515880.2659485622445836No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAA510260.26305131691657213No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC472000.24332736562658652No Hit
ATATAAAGAATGGTACAGCAATTAATATAAAAAAAAAAAA465410.23993006194124922No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAA456580.23537798431734505No Hit
TGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC439020.22632538147750852No Hit
ATGTACACGGAGTCGACCCGCAACGCGATAAAAAAAAAAA428650.22097939677083964No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA428000.22064430611902336No Hit
ATAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT415150.21401982169465553No Hit
ATGTACACGGAGTCGACCCGCAACGCGTAAAAAAAAAAAA394870.20356499335798775No Hit
GTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT367090.18924373442343992No Hit
AATACTGAATATCTAAATATTATTATTAAAAAAAAAAAAA355530.18328427606190745No Hit
AGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC354790.1829027882429166No Hit
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT352420.1816809961739865No Hit
TCGTCAATTAAAATGTTACGTGAGTTGGGTTAAAAAAAAA342870.17675774121268584No Hit
AATACTGAATATCTAAATATTATTATTACAAAAAAAAAAA341240.1759174369627466No Hit
TTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT338870.17469564489381648No Hit
ATGTACACGGAGTCGACCCGCAACGAGAAAAAAAAAAAAA338120.17430900183402848No Hit
TGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT322070.1660348403545651No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA319990.164962550268753No Hit
ATTTTAAAACCTAAAGGTAAACCTTTATAAAAAAAAAAAA316880.1633592703808321No Hit
ATAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG313090.16140543411870334No Hit
TGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAA307820.15868862221859295No Hit
ATATATAAAGAATGGTACAGCAATTAATATAAAAAAAAAA299850.1545798953032457No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGGAAAAAAAA289680.1493370154125203No Hit
TATATAAAGAATGGTACAGCAATTAATATAAAAAAAAAAA289450.14921844487418534No Hit
AATACTGAATATCTAAATATTATTATTACTAAAAAAAAAA273150.14081540237479262No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAATAAAAAAAAA270790.13959876554665968No Hit
TGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAA258440.13323204316215048No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC246400.12702513324235365No Hit
TGTTAATAGATAATATACGGATCTTAAAAAAAAAAAAAAA239570.12350410377788418No Hit
TTTGCTCGAATATATTAGCATGAAAAAAAAAAAAAAAAAA239350.12339068848034637No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC232820.1200243162397921No Hit
GCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT224290.11562689583980315Illumina Paired End PCR Primer 2 (95% over 21bp)
TTTGTTAATAGATAATATACGGATCTTAAAAAAAAAAAAA218680.11273480575258886No Hit
AGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT218560.11267294286302278No Hit
GTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT211720.10914675815775614Illumina Paired End PCR Primer 2 (95% over 21bp)
GCAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG207790.10712074852446697No Hit
TGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT207280.10685783124381114Illumina Paired End PCR Primer 2 (95% over 21bp)
TTAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG204990.10567728110125842No Hit
GGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC203360.10483697685131914No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAATAAAAAAAA196470.10128501594206664No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACAAAAAA196320.10120768733010903No Hit
TGTACACGGAGTCGACCCGCAACGTGAAAAAAAAAAAAAA195680.10087775191908994No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)