FastQCFastQC Report
Mon 25 Sept 2023
SRR387875.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387875.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19800933
Total Bases792 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2598791.3124583573915432No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAA1434890.7246577724393087No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAA1378170.6960126575853774No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAA1237220.6248291431519919No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA1186090.5990071275934321No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA948180.47885622359310037No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA922080.46567502652526527No Hit
TGTTAATAGATAATATACGGATCTTAATAAAAAAAAAAAA494470.24972055609702834No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAA483310.2440844580404368No Hit
AGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAA432060.2182018392769674No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGGTAAAAAAAAAA376270.19002639926108533No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAA374930.18934966347292828No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAA343100.17327466337066036No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA329780.1665477076256962No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA325330.1643003387769657No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAA321650.1624418404930717No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC320290.16175500417076305No Hit
TTTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAA320060.1616388480280197No Hit
ATGTACACGGAGTCGACCCGCAACGTGAAAAAAAAAAAAA312500.15782084611871572No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC302330.15268472450262824No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAA295630.14930104556184295No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAA294760.14886167232624847No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA294600.1487808680530357No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC275310.1390389028638196No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGGTAAAAAAAAAAA274760.13876113817465066No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA273560.1381551061255548No Hit
TGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAA272630.1376854312875055No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAA265610.13414014380029468No Hit
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC247440.12496380852356805No Hit
AGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAAAAAA247130.12480725024421829No Hit
TGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC242580.12250937872472979No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAA237020.1197014302305856No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAA230900.11661066678019669No Hit
GTTAATAGATAATATACGGATCTTAATAAAAAAAAAAAAA215910.10904031643357412No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAA205110.1035860279917113No Hit
ATATAAAGAATGGTACAGCAATTAATATAAAAAAAAAAAA205080.10357087719048391No Hit
AGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAAAAAA203190.1026163767131579No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)