FastQCFastQC Report
Mon 25 Sept 2023
SRR387876.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387876.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20362112
Total Bases814.4 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA2055491.00946797660282No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA1788170.8781849348436941No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAA1387620.6814715487273619No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1269440.6234323826526442No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA1201980.5903022240522005No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA989390.48589753361537347No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAA842450.4137340959523256No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAA714280.3507887590442485No Hit
AAATAGATAATATACGGATCTTAATAAAAAAAAAAAAAAA672950.3304912574884177No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA545960.268125428246343No Hit
TTTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAA543500.2669173020951854No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAA525360.2580085995008769No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA515860.25334307168136583No Hit
ATGTACAGTAAAATTGTAAGAAAAAAAAAAAAAAAAAAAA512810.25184519169720704No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA504490.24775917154369842No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAA465250.2284880861081601No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA425980.20920226742687595No Hit
ATTTTAAAACCTAAAGGTAAACCTTTATAAAAAAAAAAAA402410.19762684735257324No Hit
TGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAA381200.18721044261027542No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAA339510.16673614210549476No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAA335620.16482573124045286No Hit
AATAGATAATATACGGATCTTAATAAAAAAAAAAAAAAAA328300.16123081927847172No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAAA326770.16047942374543467No Hit
GGCGCCAGTGAAATACCACTACCTTTAAAAAAAAAAAAAA315430.15491025685351303No Hit
TTTTAAAACCTAAAGGTAAACCTTTATAAAAAAAAAAAAA309550.15202254068733145No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAA303920.14925760156903173No Hit
TGTACAACGGGGTATTGTAAGCAAAAAAAAAAAAAAAAAA291470.14314330458451463No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA288770.14181731246739043No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAA281830.1384090216181897No Hit
AATACTGAATATCTAAATATTATTATTACTTAAAAAAAAA274520.13481902073812382No Hit
ATTTTAAAACCTAAAGGTAAACCTTTAAAAAAAAAAAAAA262080.12870963483552197No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA249970.12276231463612419No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAA241670.11868611664644611No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAA210390.10332425241546654No Hit
AGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAA206770.10154644076213705No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)