FastQCFastQC Report
Mon 25 Sept 2023
SRR387877.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387877.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19268359
Total Bases770.7 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA2152081.1168984343710848No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA1584800.8224883084231511No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA1284910.6668497301716249No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAA1245410.6463498007277112No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1158950.6014783095955395No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA1076710.5587969375077556No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAA866190.44954009835502856No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAA760320.39459509758978434No Hit
AAATAGATAATATACGGATCTTAATAAAAAAAAAAAAAAA640880.3326074628358336No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA609860.31650853090291703No Hit
TTTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAA567820.29469037814792637No Hit
ATGTACAGTAAAATTGTAAGAAAAAAAAAAAAAAAAAAAA537470.27893916653722306No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA476840.24747307230470428No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAA453920.2355779233716789No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA444610.2307461678495818No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAA436490.2265320051385798No Hit
TGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAA416410.21611077518329402No Hit
ATTTTAAAACCTAAAGGTAAACCTTTATAAAAAAAAAAAA390150.20248221449475795No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA355780.1846446809507753No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA326570.16948511287339No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAA318120.16509968492905908No Hit
TTTTAAAACCTAAAGGTAAACCTTTATAAAAAAAAAAAAA304630.1580985697847959No Hit
AATAGATAATATACGGATCTTAATAAAAAAAAAAAAAAAA300290.1558461724737431No Hit
GGCGCCAGTGAAATACCACTACCTTTAAAAAAAAAAAAAA294470.15282567654048795No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAA293710.15243124751827597No Hit
TGTACAACGGGGTATTGTAAGCAAAAAAAAAAAAAAAAAA291920.15150226337385556No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAAA289850.15042796327388336No Hit
AATACTGAATATCTAAATATTATTATTACTTAAAAAAAAA279470.14504089320735616No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAA272390.14136647547411796No Hit
ATTTTAAAACCTAAAGGTAAACCTTTAAAAAAAAAAAAAA249250.12935714971887333No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAA248100.12876031632999987No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA238470.12376248543012926No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAA218620.11346062215261818No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA208360.10813583035275605No Hit
TTTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAA204270.10601317943058877No Hit
TTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAA195250.10133192971959884No Hit
TTTTAAAACCTAAAGGTAAACCTTTAAAAAAAAAAAAAAA194990.1011969934751579No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)