FastQCFastQC Report
Mon 25 Sept 2023
SRR387879.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387879.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10192759
Total Bases407.7 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAA982410.9638312845422912No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAA884890.8681555209928931No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC880410.8637602439143317No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA725190.711475666205784No Hit
ATGTACACGGAGTCGACCCGCAACGTGAAAAAAAAAAAAA703030.6897347420850429No Hit
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC667060.6544449839341832No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA617170.6054984720035076No Hit
ATAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT513770.5040539072884976No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC491210.48192054771431364No Hit
TGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC474630.4656540981691022No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA460620.45190904641226187No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAA446560.4381149402237412No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC442450.4340826659396146No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC426310.4182478953931904No Hit
ATAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG393410.38597007934750543No Hit
TTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT387360.38003449311418036No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAA387350.3800246822278443No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAA379490.37231332556768976No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC370830.36381709800064926No Hit
TGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT357240.3504841034699241No Hit
AGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC336650.3302834885039468No Hit
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT330750.3244950655656628No Hit
TGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT319340.31330084425620186Illumina Paired End PCR Primer 2 (95% over 21bp)
ATAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT315660.30969043808452645Illumina Paired End PCR Primer 2 (95% over 21bp)
GTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT313440.30751242131791795No Hit
GCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT272740.2675821139300949Illumina Paired End PCR Primer 2 (95% over 21bp)
AGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAA269670.26457017182492004No Hit
TTAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG263180.2582029065928077No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA262640.2576731187306597No Hit
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT254240.24943197420835714No Hit
GTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT249490.24477180319872177Illumina Paired End PCR Primer 2 (95% over 21bp)
GGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAA241040.23648160424473885No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAA240630.23607935790495976No Hit
CGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC234930.23048715269339734No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAA231720.22733785817951746No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA230780.22641563486392643No Hit
TGAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG228680.22435534873335078Illumina Paired End PCR Primer 2 (95% over 22bp)
AGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT223990.2197540430417319No Hit
GGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC223420.21919482252057565No Hit
TTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT219240.2150938720320965Illumina Paired End PCR Primer 2 (95% over 21bp)
GCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG216320.21222909322196276Illumina PCR Primer Index 2 (95% over 22bp)
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT212100.20808889918813933No Hit
TCAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG207860.20392908338164378No Hit
CCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC206100.2022023673864947No Hit
GCAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG204280.20041678607332913No Hit
CGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT203790.19993605264286146Illumina PCR Primer Index 1 (95% over 21bp)
CGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT200120.19633545735752211No Hit
ACAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG198910.19514834011085713No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA196520.19280353827653532No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAA189720.1861321355680047No Hit
ATGTACACGGAGTCGACCCGCAACGCCAAAAAAAAAAAAA188790.1852197231387498No Hit
AGATTTGAAATATTTATTATATAGATTTAAAAAAAAAAAA184320.18083425694652447No Hit
AGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT182030.17858756397556344Illumina Paired End PCR Primer 2 (95% over 21bp)
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAA179100.17571297427909363No Hit
GGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT176270.17293649344598455No Hit
CTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC171540.16829594420902133No Hit
TGAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG171530.16828613332268524No Hit
GTAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG170050.16683412214494622Illumina PCR Primer Index 1 (95% over 22bp)
CGAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG170010.16679487859960193Illumina Paired End PCR Primer 2 (95% over 22bp)
ATAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG163950.1608494814799408Illumina Paired End PCR Primer 2 (95% over 22bp)
GGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT161030.15798470266980708Illumina Paired End PCR Primer 2 (95% over 21bp)
TCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT160360.1573273732852901Illumina Paired End PCR Primer 2 (95% over 21bp)
ATGTACACGGAGTCGACCCGCAACGCGGAAAAAAAAAAAA158320.1553259524727309No Hit
ATAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCT156940.1539720501583526No Hit
GTAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG155710.15276531113901545No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAA154050.1511367040072271No Hit
ACAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT150930.14807570747037185Illumina Paired End PCR Primer 2 (95% over 21bp)
ATGTACACGGAGTCGACCCGCAACGCTAAAAAAAAAAAAA149200.14637842413423097No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAA144820.14208125591903037No Hit
AGAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG137880.1352725008017947No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAA135950.13337899973893233No Hit
CCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT134340.13179944703882432No Hit
AGAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG132810.13029838142940492Illumina PCR Primer Index 2 (95% over 22bp)
GGAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG125590.12321492149475917Illumina Paired End PCR Primer 2 (95% over 22bp)
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAA119060.11680841271730254No Hit
CTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT114210.11205013284430643No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA110580.1084887811043114No Hit
TTAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG108970.10690922840420343Illumina PCR Primer Index 1 (95% over 22bp)
TTTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAA106540.10452518302453732No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA104410.10243546423495345No Hit
CCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT103260.10130721230630489Illumina PCR Primer Index 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)