FastQCFastQC Report
Mon 25 Sept 2023
SRR387880.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387880.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14714524
Total Bases588.5 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAA2164881.4712538441610479No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAA1887321.2826238891587658No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA1862421.2657018331004115No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAA1821961.238205191007198No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAA1821321.2377702465944531No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAA1646351.1188605217538807No Hit
ATGTACACGGAGTCGACCCGCAACGTGAAAAAAAAAAAAA1556641.057893547898661No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA1446210.9828452486808272No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA1094880.744081154103252No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA908290.6172744697687809No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA716060.4866348377969957No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAA652730.4435957289546029No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAA609300.4140806729459954No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAA578200.39294509288917534No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC514270.3494982236598343No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA498400.3387129614250519No Hit
TGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC498100.33850908123157775No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC458650.3116988357897272No Hit
TTTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAA456110.3099726501516461No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAA445380.3026805352317207No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAA432400.2938593188607392No Hit
AGATTTGAAATATTTATTATATAGATTTAAAAAAAAAAAA418880.28467111814150425No Hit
TGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT407860.27718191903455386No Hit
ATGTACACGGAGTCGACCCGCAACGCCAAAAAAAAAAAAA401110.2725946146813855No Hit
GTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT399520.2715140496559726No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAA399010.2711674533270665No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC392110.26647820887716106No Hit
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC381640.2593627901249133No Hit
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT370570.2518396109857172No Hit
AGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC364480.247700843058192No Hit
ATGTACACGGAGTCGACCCGCAACGCGGAAAAAAAAAAAA359610.24439118791746167No Hit
TGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT349880.23777867364245014Illumina Paired End PCR Primer 2 (95% over 21bp)
ATGTACACGGAGTCGACCCGCAACGCTAAAAAAAAAAAAA343260.2332797173731206No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA337040.2290526013617566No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAA325600.2212779699839424No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAA314850.21397226305111872No Hit
GTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT305510.2076247930276236Illumina Paired End PCR Primer 2 (95% over 21bp)
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAA300650.2043219338933424No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAA292170.19855892042447312No Hit
GCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT289660.1968531228057394Illumina Paired End PCR Primer 2 (95% over 21bp)
TTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT276630.18799792640251226No Hit
TTTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAA268460.18244558913356626No Hit
TGAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG268040.18216015686270245Illumina Paired End PCR Primer 2 (95% over 22bp)
ATAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT263960.17938738623145403No Hit
GGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC259290.17621365121970645No Hit
AGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT253980.17260497179521403No Hit
GCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG245090.16656332206193009Illumina PCR Primer Index 2 (95% over 22bp)
GCAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG238740.1622478579667273No Hit
CGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC231520.15734114131044946No Hit
GTAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG220160.14962087798422838Illumina PCR Primer Index 1 (95% over 22bp)
GGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT220010.1495189378874913No Hit
CGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT218700.14862866104265418No Hit
CGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT207510.14102392982606846Illumina PCR Primer Index 1 (95% over 21bp)
ATAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG207150.14077927359389947No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAA206760.14051422934238308No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA206370.1402491850908667No Hit
GTAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG203050.13799291094975277No Hit
TGAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG201140.13669487371796737No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC198640.1349958721056828No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGGAAAAAAAA196540.13356871075136376No Hit
AGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT195990.13319493039666114Illumina Paired End PCR Primer 2 (95% over 21bp)
GGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT189700.12892024234015317Illumina Paired End PCR Primer 2 (95% over 21bp)
TTATCTATATAATATCTAATCTATTATTCTATAAAAAAAA189670.12889985432080575No Hit
TTAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG186120.12648727203136167No Hit
CGAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG185990.1263989239475229Illumina Paired End PCR Primer 2 (95% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGATAAAAAAAAAAA183780.12489700652226332No Hit
TCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180670.12278344851658131No Hit
ATGTACACGGAGTCGACCCGCAACGCGTAAAAAAAAAAAA179660.12209705186521833No Hit
ATGTACACGGAGTCGACCCGCAACGAGAAAAAAAAAAAAA176690.12007863794982426No Hit
AGAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG166970.11347291968126186No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAA163150.11087684521769103No Hit
TGTTAATAGATAATATACGGATCTTAATAAAAAAAAAAAA160920.1093613357795332No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAA160870.1093273557472875No Hit
GGAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG157550.10707108160617361Illumina Paired End PCR Primer 2 (95% over 22bp)
AGAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG156840.10658856514828478Illumina PCR Primer Index 2 (95% over 22bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAA155990.10601090460010804No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGT151120.10270124945937768No Hit
TTTATCTATATAATATCTAATCTATTATTCTATAAAAAAA150440.10223912102083628No Hit
ATAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT149620.10168184849200694Illumina Paired End PCR Primer 2 (95% over 21bp)
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGT147550.10027507515703532No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)