FastQCFastQC Report
Mon 25 Sept 2023
SRR387881.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387881.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20115784
Total Bases804.6 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA1797390.8935222211572762No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA1207110.6000810110110548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1153220.5732911031456691No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAA902370.44858803415268333No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA863680.42935438161396045No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA792990.39421282312436845No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAA781560.3885307179675423No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAA661590.32889098431361163No Hit
TTTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAA580200.2884302197716977No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC530810.26387736118065297No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAA468240.23277243382609397No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC452880.22513663896967673No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAA443110.22027975643405198No Hit
TGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC422910.2102378908025658No Hit
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC405800.20173213233946038No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA403780.20072794577631176No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC382360.19007959123044868No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAA360160.17904348147703314No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAA340890.1694639393622441No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAA334960.16651600554072366No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAA321670.15990925335050327No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAA316590.15738387328080278No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310020.15411778134026494No Hit
ATAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT299870.14907199242147362No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA291990.14515467058107206No Hit
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT290620.14447361335755046No Hit
ATTTTAAAACCTAAAGGTAAACCTTTAAAAAAAAAAAAAA290020.14417534012097166No Hit
TGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT285220.1417891542283413No Hit
AGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC284670.14151573709481072No Hit
TGTTAATAGATAATATACGGATCTTAATAAAAAAAAAAAA280300.13934331368839514No Hit
ATTTTAAAACCTAAAGGTAAACCTTTATAAAAAAAAAAAA276830.13761830013684775No Hit
GTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT275940.1371758615025892No Hit
TTTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAA275640.13702672488429982No Hit
GCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT257290.12790453506559823Illumina Paired End PCR Primer 2 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA253500.12602044245454216No Hit
TGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT242420.12051233001905369Illumina Paired End PCR Primer 2 (95% over 21bp)
GGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC242060.12033336607710642No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAA234830.1167391735763319No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAA234830.1167391735763319No Hit
ATAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG231300.11498433270112664No Hit
GTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT229230.11395529003492978Illumina Paired End PCR Primer 2 (95% over 21bp)
CGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC228890.1137862685342018No Hit
TTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT227420.11305549910458373No Hit
ATGTACACGGAGTCGACCCGCAACGTGAAAAAAAAAAAAA213980.10637417860521867No Hit
TGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAA208710.10375434534393489No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC208650.103724518020277No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)