FastQCFastQC Report
Mon 25 Sept 2023
SRR387882.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387882.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18775879
Total Bases751 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA2748721.4639634181707284No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA1984981.0571968428215797No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC1131240.6024964263989985No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1119620.5963076349181841No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA1055630.5622266739149736No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA982710.5233896106808102No Hit
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC771730.4110220352400013No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC753750.40144591898999776No Hit
TGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC750680.3998108424111596No Hit
TGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAA728290.38788596794855784No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC636750.3391319255945354No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAA628700.33484450980963393No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA627720.33432256354016765No Hit
ATAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT627170.33402963451138556No Hit
AGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC585780.3119853935999481No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAA573650.3055249770197177No Hit
TGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT544070.28977072125358283No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAA512610.2730151808072474No Hit
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT499850.266219227339503No Hit
GTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT489800.26086661508630304No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAA464190.24722677430974072No Hit
TGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT456170.24295533647186374Illumina Paired End PCR Primer 2 (95% over 21bp)
ATAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG452800.24116048042278077No Hit
TTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT450570.23997278636062794No Hit
TTTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAA442010.2354137454763103No Hit
GGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC412350.21961688185144357No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAA410860.21882331048256118No Hit
GCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT409190.2179338714315319Illumina Paired End PCR Primer 2 (95% over 21bp)
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC402730.21449328683892777No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC392620.20910871869167882No Hit
GTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT383290.20413957716706632Illumina Paired End PCR Primer 2 (95% over 21bp)
CGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC362430.19302957800271295No Hit
AGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT355400.1892854124166437No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAA339210.18066264700576734No Hit
ATAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT334210.1779996558350211Illumina Paired End PCR Primer 2 (95% over 21bp)
GGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT315960.16827973806179727No Hit
CGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT315770.1681785443973089No Hit
CGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT315220.1678856153685268Illumina PCR Primer Index 1 (95% over 21bp)
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAA313690.16707074007027847No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAA299370.1594439333572612No Hit
GCAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG297210.1582935211714988No Hit
TTAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG283970.15124192055136274No Hit
GGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT283040.15074660419360394Illumina Paired End PCR Primer 2 (95% over 21bp)
AGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT272600.14518627862908576Illumina Paired End PCR Primer 2 (95% over 21bp)
GCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG259970.13845956293178072Illumina PCR Primer Index 2 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA254340.13546103487352043No Hit
TGAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG252540.1345023580520518No Hit
CTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC250870.13361291900102254No Hit
TGAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG242100.1289420324875336Illumina Paired End PCR Primer 2 (95% over 22bp)
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGT231900.12350953049921126No Hit
GTAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG229900.12244433403091275No Hit
TTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT229100.12201825544359336Illumina Paired End PCR Primer 2 (95% over 21bp)
TTTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAA226770.12077730155802559No Hit
AGAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG224440.11953634767245783No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAA223930.11926472257304173No Hit
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT222100.11829006780454858No Hit
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT214220.1140931937194525No Hit
GTAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG205630.10951817488811043Illumina PCR Primer Index 1 (95% over 22bp)
CGAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG205200.10928915764742625Illumina Paired End PCR Primer 2 (95% over 22bp)
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGT201170.10714278676380477No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACAAAAAAAA198070.1054917322379421No Hit
CCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC196420.10461294515159583No Hit
ACAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG190140.10126822824113853No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA190070.1012309463647481No Hit
TTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAAA188300.1002882474903039No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)