FastQCFastQC Report
Mon 25 Sept 2023
SRR387883.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387883.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18383469
Total Bases735.3 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC24

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA3708712.0174157554267915No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA2299171.2506725471672402No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA1266000.6886621888393317No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1256630.6835652182947625No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAA1168000.6353534254062713No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA1161950.6320624252147405No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAA848650.4616375723210891No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAA826510.44959414352100796No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA679970.3698812231793684No Hit
TTTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAA674420.36686220647474094No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAA599820.3262822702287583No Hit
TGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAA599760.3262496322103298No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAA553760.30122715141521983No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAA545280.2966143114773387No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAA471760.2566218595630672No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAA448390.24390935138520375No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAA431220.23456943844494205No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAA401430.2183646622952393No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAA369990.20126234063875542No Hit
TTTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAA321290.17477114901436722No Hit
TTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAAA319480.173786568791777No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA288600.15698886864062492No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA288550.15696167029193456No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAA279170.15185926007762737No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACAAAAAAAA227740.12388303861474675No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGAAAAAAA207890.11308529418468298No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA199880.10872811872449102No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC184780.10051421742000924No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)