FastQCFastQC Report
Mon 25 Sept 2023
SRR387884.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387884.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16250977
Total Bases650 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAA2267871.395528404230712No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAA1824611.1227694187247943No Hit
ATGTACACGGAGTCGACCCGCAACGTGAAAAAAAAAAAAA1742371.0721632305553075No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAA1629791.0028873956316595No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1485890.9143388732874338No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAA723780.44537629952956065No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA689690.4243990992049278No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA686880.4226699723961212No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAA527890.32483585448431807No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAA524490.3227436725804239No Hit
AGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAA518980.31935310720087784No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA432390.26607015688964425No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAA397880.24483451056511862No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAA394220.24258233828033846No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAA392030.24123472699518314No Hit
ATGTACACGGAGTCGACCCGCAACGCTAAAAAAAAAAAAA385860.23743803218723405No Hit
ATGTACACGGAGTCGACCCGCAACGCGGAAAAAAAAAAAA358020.2203067544800537No Hit
ATGTACACGGAGTCGACCCGCAACGCCAAAAAAAAAAAAA343420.21132267924568474No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA340080.20926741819891811No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC327500.2015263451545098No Hit
AGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAAAAAA300220.1847396621138532No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC274240.16875293097762678No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA268120.1649870035506173No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAA260460.16027344079066755No Hit
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT234620.14437285832107202No Hit
GTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT225360.13867473937105443No Hit
TGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC222730.13705637513363045No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAA221020.13600413070549544No Hit
AGATTTGAAATATTTATTATATAGATTTAAAAAAAAAAAA217400.13377657232546697No Hit
AGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAAAAAA206580.12711851109013322No Hit
ATGTACACGGAGTCGACCCGCAACGCGATAAAAAAAAAAA202160.12439867461507084No Hit
ATGTACACGGAGTCGACCCGCAACGCGTAAAAAAAAAAAA200710.12350642056782185No Hit
TTTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAA197660.12162961033050504No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC196780.12108810442596774No Hit
AGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC186210.11458388009533212No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAA180910.1113225377157324No Hit
ATGTACACGGAGTCGACCCGCAACGAGAAAAAAAAAAAAA180860.1112917703347928No Hit
TGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT177630.10930419752609336No Hit
GTTTCTTTACTTATTCAATGAAGCGGAAAAAAAAAAAAAA169190.10411066362348553No Hit
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC166390.10238769029086682No Hit
GGAATGCAGCTCTAAGTGGGTGGTAAAAAAAAAAAAAAAA165690.10195694695771214No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA165270.10169850095781932No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)