FastQCFastQC Report
Mon 25 Sept 2023
SRR387886.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387886.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13362439
Total Bases534.4 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA2710182.028207574979388No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA2411861.8049549187839136No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAA1677661.2555043282143328No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA1363731.0205696729466829No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA808100.6047548654852606No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAA543740.4069167312943393No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGGAAAAAAAAAAAA517580.38733946699401206No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA485580.3633917430792387No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA483200.36161063111307745No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAA428110.32038312766105054No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAAA415490.31093874404216176No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAA341020.25520790029425017No Hit
CAAGACTGTAAATCTTGAGAAAAAAAAAAAAAAAAAAAAA328580.24589822262238203No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAA328460.24580841865770164No Hit
TTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAA312890.23415635424041972No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAGAAAAAAAAAAA297560.22268389775249864No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGGTAAAAAAAAAAA295490.2211347793617617No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAA282400.21133866354787473No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACAAAAAAAA275340.20605519695917787No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAA275220.2059653929944975No Hit
TGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAA264160.19768846091645395No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA257850.1929662691070096No Hit
ATAAATCATTTGTATACGACTTAGACAAAAAAAAAAAAAA253610.18979319568830214No Hit
TTTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAA207990.15565272178230338No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAA207000.15491183907369008No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA199440.14925418929882486No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGAAAAAAAAAAAAA195120.14602124657033047No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAA193930.1451306905872498No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCAAAAAAAAAA184910.13838042590877309No Hit
ATAAATCATTTGTATACGACTTAGATGAAAAAAAAAAAAA175740.13151790627444587No Hit
ATAAATCATTTGTATACGACTTAGAAAAAAAAAAAAAAAA174240.13039535671594085No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAAA169790.12706512635904268No Hit
ATAAATCATTTGTATACGACTTAGGTAAAAAAAAAAAAAA167400.12527653072915806No Hit
AAAGATTGGAAATCTTTTGAAAAAAAAAAAAAAAAAAAAA165510.12386211828544175No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACAAAAAAA162600.12168437214194205No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCAAAAAAAAAAAAA154720.11578724512792912No Hit
TTTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAA153640.11497900944580552No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCGAAAAAAAA153230.11467217923314749No Hit
TGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAAA148370.11103511866359128No Hit
TTGATGATTCATAATAACTTTTCGAATCGCGAAAAAAAAA142330.10651498577467781No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)