FastQCFastQC Report
Mon 25 Sept 2023
SRR387887.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387887.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11961488
Total Bases478.4 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA4670543.9046479835953516No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA3950813.3029419082308156No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3527602.9491314124129038No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAA2561512.141464339553741No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA1285171.074423182132524No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1146150.958200183789843No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA912900.7631993611497164No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA827830.6920794469718149No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAA773160.6463744310072459No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA725220.6062958053379311No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA563690.4712540780879436No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAA520820.43541405550881296No Hit
TTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAA471170.3939058418149983No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACAAAAAAAA453210.3788909874758057No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA412740.3450574042293066No Hit
AGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA406950.34021686933933304No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAAA390020.3260631118803948No Hit
TTTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAA376730.31495245407594774No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAA336060.28095166755172934No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320380.2678429305785367No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACAAAAAAA315550.26380497142161574No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAA310110.259257042267651No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA305270.2552107229468441No Hit
TGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAA296030.2474859315162127No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAGAAAAAAAAAAA290310.24270391777344089No Hit
ACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAAAAAA287050.2399785043466164No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAA286520.23953541566066028No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA247710.20708961961923134No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGGTAAAAAAAAAAA242140.20243300833474898No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCTAAAAAAAA239830.20050181047709115No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAA239740.20042656900211747No Hit
TGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAAA228570.19108826594149492No Hit
ATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA221580.1852445113852056No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCGAAAAAAAA201120.16813961607452182No Hit
AAAGATTGGAAATCTTTTGAAAAAAAAAAAAAAAAAAAAA199940.16715311673597802No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA191930.16045662546332026No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCAAAAAAAAAA185090.15473827336532042No Hit
TTTCCTTGTCTATGTTCCTTGGAACAGGACGTCAAAAAAA182320.15242250796890822No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAAAA176330.14741476980121537No Hit
TTTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAA164710.13770025936572441No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGAAAAAA159500.13334461398113678No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAAA159200.13309380906455787No Hit
AATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAAAA156880.13115425104301404No Hit
TTTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAA155360.12988350613234742No Hit
TTGATGATTCATAATAACTTTTCGAATCGCTAAAAAAAAA149440.1249342891118563No Hit
TTGATGATTCATAATAACTTTTCGAATCGCGAAAAAAAAA147990.12372206534839143No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAA143860.12026931766348803No Hit
AGTTTTGGTGGCAGGATAAATCCAAAAAAAAAAAAAAAAA141790.11853876373909332No Hit
TGATGATTCATAATAACTTTTCGAATCGCACAAAAAAAAA138840.11607251539273375No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAAA138770.11601399424553199No Hit
TTGATGATTCATAATAACTTTTCGAATCGCCAAAAAAAAA138390.11569630801786535No Hit
CAAGACTGTAAATCTTGAGAAAAAAAAAAAAAAAAAAAAA137130.11464292736823378No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAA135550.11332202147425136No Hit
TGGGCCAGCATCAGTTTTGGTGGCAAAAAAAAAAAAAAAA134430.11238568311902333No Hit
TTAAGGCAAAAGATTGGAAATCTTTTGAAAAAAAAAAAAA132450.11073037066960231No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCGTAAAAAAA131910.11027892181976022No Hit
TTTGATGATTCATAATAACTTTTCGAATCGTAAAAAAAAA130810.10935930379230409No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAGAAAAAAA130800.10935094362841814No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGAAAAAAA127410.10651684807107611No Hit
GATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAA127290.10641652610444453No Hit
TTGATGATTCATAATAACTTTTCGAATCGCGTAAAAAAAA125530.10494513726051476No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGAGAAAAAAAAAAAA122450.10237020678363763No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)