FastQCFastQC Report
Mon 25 Sept 2023
SRR387888.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387888.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7045423
Total Bases253.6 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAA1480632.101548764353822No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAA866751.2302313147131123No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAA339550.48194409334968247No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAA337760.4794034368128074No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAA311830.4425994010579634No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAA303060.43015160338846936No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAA258200.36647906023527616No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAA239120.33939764865785915No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAA182340.25880632007474924No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAA154800.2197171127979115No Hit
ATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAA141830.2013079981145206No Hit
AATACTGAATATCTAAATATTATTATTACAAAAAAA138670.19682281674216012No Hit
ATAAATCATTTGTATACGACTTAGAAAAAAAAAAAA136100.19317505847413277No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAA125470.17808724898419867No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA118550.16826526952320675No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAAAAAA112940.16030265322607315No Hit
TGTTAATAGATAATATACGGATCTTAATAAAAAAAA111070.15764844779369527No Hit
TGAGATTAAGCCTTTGTTGTCTGATTTGTAAAAAAA110590.1569671544206785No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAA102870.1460096860046586No Hit
AATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAA102460.1454277479152068No Hit
AATACTGAATATCTAAATATTATTATTACTAAAAAA96140.13645738517048586No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAA95440.1354638323348364No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAA91430.1297721939477587No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAA89380.12686250350049955No Hit
ATATATAAAGAATGGTACAGCAATTAATATAAAAAA87940.1248186233814492No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACTTTGAAAAA82070.1164869731739315No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCACTAAAA81890.1162314881590502No Hit
TTGAGATTAAGCCTTTGTTGTCTGATTTGTAAAAAA79190.11239921293583083No Hit
TTTGCTCGAATATATTAGCATGGAAAAAAAAAAAAA77720.11031275198096692No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAA75880.10770112738440261No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAA75810.10760177210083766No Hit
ATAAATCATTTGTATACGACTTAGACAAAAAAAAAA74370.10555789198178733No Hit
GTTAATAGATAATATACGGATCTTAATAAAAAAAAA72430.10280433126584451No Hit
ATCGTGAGTTCACCCCTCACTGGGGTCGCCAAAAAA72180.10244949096739828No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAA70710.10036303001253438No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)