FastQCFastQC Report
Mon 25 Sept 2023
SRR387889.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387889.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12665880
Total Bases506.6 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA1368511.0804697344361387No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA1288981.0176789926953358No Hit
TTTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAA770240.6081219781017979No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA759680.5997846182026041No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAA675180.5330699485546997No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA477110.3766891838545762No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAA455120.35932757929176656No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAA370360.29240763373725315No Hit
TTTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAA362960.28656516562607576No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCAAAAAAAAAAAAAA362960.28656516562607576No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAA336880.2659744131477639No Hit
TTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAAA329320.2600056214017502No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA318420.2513998237785294No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA313650.24763380041497315No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA294020.23213546946599845No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAAA238570.188356434768054No Hit
ATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA225410.17796631580277092No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAA221540.17491086288516866No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGGAAAAAAAAAAAA217550.1717606672414392No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAA215270.16996055544502237No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAA209780.16562607572470292No Hit
TGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAAAA207270.16364437370320895No Hit
AGTTTTGGTGGCAGGATAAATCCAAAAAAAAAAAAAAAAA206570.16309170780080026No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAA200560.15834667626726293No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAA187780.14825657593471595No Hit
CGCGCAGGTTCGAGTCCTGCAGTTGTCGCCAAAAAAAAAA175460.13852965605232326No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACAAAAAAAA173520.1369979819799335No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAA172080.13586106926640706No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAGAAAAAAAAAAA166750.13165291318092387No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGGTAAAAAAAAAAA154790.12221022147691277No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCAAAAAAAAAAAAAA153060.12084434717524561No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAA151330.11947847287357846No Hit
TTTGCTCGAATATATTAGCATGAAAAAAAAAAAAAAAAAA147250.11625722018525361No Hit
ACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAAAAAA143760.11350178590038751No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAA142200.11227013046073388No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAA139760.11034369502948078No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAA139500.11013841912287184No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGCCAAAAAAAAAAA136080.1074382514282466No Hit
TGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAA136010.10738298483800574No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAA135870.107272451657524No Hit
CAAGACTGTAAATCTTGAGAAAAAAAAAAAAAAAAAAAAA135620.10707507097809231No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAA134500.10619080553423844No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)