FastQCFastQC Report
Mon 25 Sept 2023
SRR387890.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387890.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10210064
Total Bases408.4 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA2130622.08678417686706No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA2066992.024463313844066No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAA1122351.0992585354998754No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAA820570.8036874205685685No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA811720.7950195023263322No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA559510.5479985238094491No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA462660.45314113603989165No Hit
ATAAATCATTTGTATACGACTTAGACAAAAAAAAAAAAAA397050.3888810099525331No Hit
TTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAA304110.2978531770222009No Hit
ATAAATCATTTGTATACGACTTAGAAAAAAAAAAAAAAAA293510.28747126364731895No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAAA290650.28467010588768105No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA284460.27860746024706606No Hit
CAAGACTGTAAATCTTGAGAAAAAAAAAAAAAAAAAAAAA275940.2702627525155572No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAA270030.2644743460961655No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAA257140.2518495476619931No Hit
ATAAATCATTTGTATACGACTTAGATGAAAAAAAAAAAAA252630.24743233734871786No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA245530.2404784142391272No Hit
ATAAATCATTTGTATACGACTTAGGTAAAAAAAAAAAAAA241310.2363452374049761No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAA225460.22082133863215747No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAA220330.21579688432903066No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGGAAAAAAAAAAAA212760.20838263109810087No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAA201680.19753059334397904No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAA200740.19660993310130084No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAGAAAAAAAAAAA198560.19447478487891948No Hit
TTTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAA195100.1910859716452316No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGGTAAAAAAAAAAA184100.18031228795431645No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA156740.1535151983376402No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA144900.14191879698305515No Hit
ATCGTGAGTTCACCCCTCACTGGGGTCGCCAAAAAAAAAA136420.13361326628314962No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACAAAAAAAA136190.13338799835143053No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCGAAAAAAAA129750.12708049626329473No Hit
TGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAAA128420.12577785996248406No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA123540.12099826210687807No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCAAAAAAAAAA121900.11939200381114164No Hit
TGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAA121050.11855949188957092No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAA118090.11566039155092465No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA116110.11372112848655994No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCTAAAAAAAA113850.1115076262009719No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAAA109360.10711000440349835No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)