FastQCFastQC Report
Mon 25 Sept 2023
SRR387892.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387892.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5445846
Total Bases196 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAA422200.7752698111551447No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAA352830.647888317076906No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAA346080.635493548660759No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAA336520.6179388840595199No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAA275210.5053576616011544No Hit
ATAAATCATTTGTATACGACTTAGAAAAAAAAAAAA256130.47032178287817905No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAA183430.33682553638130786No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAA144060.2645319019303888No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA126490.23226877880865526No Hit
ATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAA125250.22999181394405938No Hit
AATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAA120440.2211593937838125No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAA101940.18718854701363205No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAA100380.18432397831301142No Hit
ATAAATCATTTGTATACGACTTAGACAAAAAAAAAA89480.16430872264841864No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAA87750.1611319894099099No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAA82500.15149161397512895No Hit
ATAAATCATTTGTATACGACTTAGATGAAAAAAAAA76260.14003333917264646No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAA68390.12558195733041294No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAA65820.12086276402233923No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAA65760.12075258830308458No Hit
ATAAATCATTTGTATACGACTTAGGTAAAAAAAAAA63700.11696988860867531No Hit
TCAAAAAAAAAAAAAAAAAAAATTGTTTGGGGTTTT58050.10659500837886345No Hit
TTTGCTCGAATATATTAGCATGGAAAAAAAAAAAAA57430.1054565259465655No Hit
ACAAAAAAAAAAAAAAAAAAAATTGTTTGGGGTTTT57310.10523617450805622No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGGTAAAAAAA55490.10189417769066551No Hit
ATAAAAAAAAAAAAAAAAAAAATTGTTTGGGGTTTT55470.10185745245091396No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)