FastQCFastQC Report
Mon 25 Sept 2023
SRR387894.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387894.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11960989
Total Bases478.4 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA2946472.4633999747010886No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA2685782.2454497700817213No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAA1507351.2602218763013662No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA744550.6224819703454288No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA641870.5366362263187434No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA513700.42947953551332585No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA499130.41729826856290897No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAA474300.39653911561995414No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAA435140.3637993480305015No Hit
TTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAA365450.3055349352800174No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACAAAAAAAA306210.2560072582626738No Hit
TTTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAA301230.2518437229563542No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280700.23467959045861508No Hit
CAAGACTGTAAATCTTGAGAAAAAAAAAAAAAAAAAAAAA267870.22395305271161106No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAA258640.21623629952339227No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAAA253740.212139648318379No Hit
TGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAA233280.19503403940928296No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACAAAAAAA232890.19470797941541457No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAA221870.18549469446046643No Hit
ATAAATCATTTGTATACGACTTAGACAAAAAAAAAAAAAA218350.18255179400298754No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCGAAAAAAAA213940.17886480791847564No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAA208910.1746594700488396No Hit
AATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAAAA190500.15926776623571848No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCTAAAAAAAA189140.15813073651351073No Hit
TGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAAA167670.14018071582542213No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAGAAAAAAAAAAA163860.13699536050070776No Hit
ATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA156210.13059956831328914No Hit
TTGATGATTCATAATAACTTTTCGAATCGCGAAAAAAAAA152500.1274978181152077No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGGTAAAAAAAAAAA151390.12656980120958225No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGAAAAAAAAAAAAA145460.1216120172002499No Hit
ATAAATCATTTGTATACGACTTAGAAAAAAAAAAAAAAAA141780.11853534854015833No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGAAAAAA137460.11492360706961606No Hit
ATAAATCATTTGTATACGACTTAGATGAAAAAAAAAAAAA136700.11428820810720586No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAA134780.11268298967585372No Hit
TTTGATGATTCATAATAACTTTTCGAATCGTAAAAAAAAA132110.11045073279475467No Hit
ATAAATCATTTGTATACGACTTAGGTAAAAAAAAAAAAAA130720.10928862153455705No Hit
TTGATGATTCATAATAACTTTTCGAATCGCTAAAAAAAAA128470.10740750618531629No Hit
ACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAAAAAA126340.10562671698803502No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAGAAAAAAA120060.10037631503548745No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)