FastQCFastQC Report
Mon 25 Sept 2023
SRR387895.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387895.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6461106
Total Bases258.4 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA4257976.590156545953588No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA3338655.167304173619811No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA542620.8398252559236763No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA511360.7914434463697082No Hit
ATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAA373910.5787089702598904No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAA320930.49671062508493125No Hit
TTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAA304040.4705695897884975No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAA301200.46617405750656316No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA287260.444598803981857No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAAA226980.351302083575165No Hit
TTTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAA211770.32776122230466426No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA188710.2920707383534646No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAA179330.2775531000420052No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAA165900.25676718506088586No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAA159640.24707844136901638No Hit
ATAAATCATTTGTATACGACTTAGACAAAAAAAAAAAAAA159360.24664507903136088No Hit
AGTTTTGGTGGCAGGATAAATCCAAAAAAAAAAAAAAAAA158800.2457783543560499No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAA156830.24272934076611652No Hit
AATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAAAA148990.2305951953117624No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA141590.21914204781658125No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGGTAAAAAAAAAAA138420.21423576706526715No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAGAAAAAAAAAAA121090.18741373380966045No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA120740.18687203088759108No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA120730.18685655366124623No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAA119250.18456592416221002No Hit
ATAAATCATTTGTATACGACTTAGAAAAAAAAAAAAAAAA115360.17854528311406745No Hit
CAAGACTGTAAATCTTGAGAAAAAAAAAAAAAAAAAAAAA115190.1782821702662052No Hit
TTGATGATTCATAATAACTTTTCTAATCGCAAAAAAAAAA112580.17424261419020212No Hit
ATAAATCATTTGTATACGACTTAGATGAAAAAAAAAAAAA107120.16579204860591978No Hit
TTGATGATTCATAATAACTTTTCGAATCGCGAAAAAAAAA102570.15874991061901786No Hit
TTGATGATTCATAATAACTTTTCGAATCGAAAAAAAAAAA102290.15831654828136235No Hit
ATAAATCATTTGTATACGACTTAGGTAAAAAAAAAAAAAA100180.15505085352260123No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAA91950.14231309624079838No Hit
TTTGATGATTCATAATAACTTTTCGAATCGAAAAAAAAAA83190.12875504596271908No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82270.12733114113899385No Hit
TTGATGATTCATAATAACTTTTCGAATCGCTAAAAAAAAA77220.11951514183484994No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAAA74980.11604824313360591No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAA74740.11567678970132977No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAA73620.11394334035070776No Hit
TTGATGATTCATAATAACTTTTCGAATCGTAAAAAAAAAA72940.11289088895925867No Hit
GGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAA70810.10959423974780788No Hit
ATCGTGAGTTCACCCCTCACTGGGGTCGCCAAAAAAAAAA69150.10702502017456454No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAA68560.106111863820219No Hit
ATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA68260.10564754702987383No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66020.1021806483286298No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)