FastQCFastQC Report
Mon 25 Sept 2023
SRR387896.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387896.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18590410
Total Bases743.6 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA16614188.936962659779962No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA15149798.149250070331963No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA1419210.7634097365254451No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA1415700.7615216662784737No Hit
TTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAA1244130.6692321471124091No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAA1005300.5407626835556613No Hit
TTTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAA982750.5286327735644345No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAA777260.41809728779515887No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA747060.4018523529066868No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAA671570.3612453948030194No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA653790.35168132386536927No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAA645770.3473672716201525No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAAA602850.32428009925547635No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC600820.3231881384003903No Hit
ATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAA541930.29151051536786976No Hit
AGTTTTGGTGGCAGGATAAATCCAAAAAAAAAAAAAAAAA505080.2716884673334262No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC451070.24263585364712237No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAA444080.23887585050571775No Hit
TTGATGATTCATAATAACTTTTCGAATCGCGAAAAAAAAA438950.23611636322168256No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAA429450.23100620158458046No Hit
AGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC405560.21815548984664676No Hit
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT398130.21415880553468158No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA386530.20791902921990427No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA376610.20258294464726706No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA373920.20113596203634024No Hit
GTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT363430.19549326776547696No Hit
TTGATGATTCATAATAACTTTTCGAATCGAAAAAAAAAAA362090.19477246601876988No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGGTAAAAAAAAAAA360550.19394408192180806No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAA354870.19088874317457227No Hit
ATAAATCATTTGTATACGACTTAGACAAAAAAAAAAAAAA347390.18686516327504343No Hit
TTTGATGATTCATAATAACTTTTCGAATCGAAAAAAAAAA339830.18279855043541265No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAGAAAAAAAAAAA335150.18028112343945077No Hit
TGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC331430.17828009172471182No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAA290130.15606433639709935No Hit
TTGATGATTCATAATAACTTTTCGAATCGCTAAAAAAAAA289880.15592985845928087No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCGAAAAAAAA289380.15566090258364393No Hit
AATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAAAA280020.15062604859172013No Hit
TTGATGATTCATAATAACTTTTCGAATCGTAAAAAAAAAA278780.1499590380201405No Hit
AGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT270340.1454190628393887No Hit
GCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT269320.1448703928530893Illumina Paired End PCR Primer 2 (95% over 21bp)
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAA261900.1408790876586369No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC261470.14064778560558913No Hit
ATAAATCATTTGTATACGACTTAGATGAAAAAAAAAAAAA259750.139722577393398No Hit
TGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT257430.1384746221304425No Hit
GGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAA254360.13682323305403163No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC253550.13638752453549974No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC243020.1307233137945855No Hit
GTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT242550.13047049527148677Illumina Paired End PCR Primer 2 (95% over 21bp)
CAAGACTGTAAATCTTGAGAAAAAAAAAAAAAAAAAAAAA237870.12795306827552486No Hit
TTTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAA235500.12667821742500568No Hit
ATAAATCATTTGTATACGACTTAGAAAAAAAAAAAAAAAA235020.1264200197843942No Hit
GCAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG234250.1260058277359133No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACAAAAAAAA231590.1245749824775247No Hit
AATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAATC219450.11804473381705945No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAAA212630.11437617567337137No Hit
TTTGATGATTCATAATAACTTTTCGAATCGTAAAAAAAAA207250.11148221045151774No Hit
ATCGTGAGTTCACCCCTCACTGGGGTCGCCAAAAAAAAAA206290.11096581517029479No Hit
ATAAATCATTTGTATACGACTTAGGTAAAAAAAAAAAAAA204180.10983082137510684No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAA202940.10916381080352719No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAA200660.10793737201062269No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAA200560.10788358083549529No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACAAAAAAA199110.10710360879614812No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCTAAAAAAAA194330.10453239062505884No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAA191920.10323602330448872No Hit
TGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT191520.10302085860397915Illumina Paired End PCR Primer 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)