FastQCFastQC Report
Mon 25 Sept 2023
SRR387897.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387897.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11365568
Total Bases454.6 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA2218291.951763431444869No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA2148621.8904642513247027No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAA1186341.0438017703998603No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA823960.7249615681328025No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA800660.7044610528923851No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA696130.6124902864511479No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA568820.5004765269980348No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAA563000.4953557974401279No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAA529110.4655376660453749No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA413770.36405571635311146No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACAAAAAAAA412950.36333423899271905No Hit
TGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAA365460.32155014162072676No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAA337720.29714309042891657No Hit
CAAGACTGTAAATCTTGAGAAAAAAAAAAAAAAAAAAAAA312960.27535799354682494No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACAAAAAAA259570.22838277858176556No Hit
ATAAATCATTTGTATACGACTTAGACAAAAAAAAAAAAAA242940.21375086577283248No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA237600.20905246442588704No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAA235800.2074687336347818No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAA231180.20340382460427847No Hit
TTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAA220390.19391023836204227No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAA213770.18808562845253313No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAAA204330.17977984030362582No Hit
ATAAATCATTTGTATACGACTTAGATGAAAAAAAAAAAAA172470.1517478053010637No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAA172020.1513518726032874No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGAAAAAA162730.14317806202030556No Hit
TTTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAA162170.14268534577418393No Hit
ATAAATCATTTGTATACGACTTAGAAAAAAAAAAAAAAAA157250.1383564816118297No Hit
TTTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAA152360.13405401296266056No Hit
ATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA151780.13354369970774888No Hit
ATCGTGAGTTCACCCCTCACTGGGGTCGCCAAAAAAAAAA151650.1334293191506135No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGAAAAAAA146890.12924123105857974No Hit
ATAAATCATTTGTATACGACTTAGGTAAAAAAAAAAAAAA145320.1278598658685602No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCGTAAAAAAA144480.12712079149937777No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAA143440.12620574704229476No Hit
TTGATGATTCATAATAACTTTTCGAATCGCGTAAAAAAAA136820.12038113713278562No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAGAAAAAAAAAAA131400.11561234775067995No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA131360.11557715373309983No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAA128030.11264725176955522No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAA127360.11205775197508827No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGGTAAAAAAAAAAA119730.10534449312168119No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA119090.10478138884039935No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCGAAAAAAAA119030.10472859781402918No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC115580.10169311379774419No Hit

[FAIL]Adapter Content

Adapter graph