FastQCFastQC Report
Mon 25 Sept 2023
SRR387899.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387899.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10793988
Total Bases431.7 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA2560462.372116774634176No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA2549322.3617962147076685No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAA1402471.2993066140151353No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1156641.0715594643981445No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA655310.6071064744559657No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA535990.496563457361635No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAA518150.48003573841290165No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAA453450.42009496397439017No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA398650.36932596182245153No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAA368220.34113434256180386No Hit
TTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAA363290.3365669852514196No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA323330.2995463771128892No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAA282710.2619143174885872No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAA262790.24345959991802843No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA259290.24021705416014916No Hit
TTTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAA257230.2383085843712259No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAA228890.2120532281488547No Hit
ATAAATCATTTGTATACGACTTAGACAAAAAAAAAAAAAA226000.20937581179449155No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAAA223510.20706897209817168No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACAAAAAAAA216590.20065799591402178No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAAAA192440.17828443018465465No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAA189570.1756255426631936No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA188070.17423588019553105No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCGAAAAAAAA186410.17269798706465117No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAA186370.17266092939884684No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA179550.16634259737920776No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCTAAAAAAAA167610.15528088413661384No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAAA164070.1520012807129302No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA161560.14967591218370818No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAGAAAAAAAAAAA159030.147332014821584No Hit
TGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAAA157030.14547913153136727No Hit
ATAAATCATTTGTATACGACTTAGATGAAAAAAAAAAAAA154950.14355213290954186No Hit
TGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAA153330.14205129744446632No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGAGAAAAAAAAAAAA151070.13995753932652139No Hit
TTGATGATTCATAATAACTTTTCGAATCGCGAAAAAAAAA150180.13913300626237493No Hit
ATAAATCATTTGTATACGACTTAGGTAAAAAAAAAAAAAA146590.1358070807564359No Hit
ATAAATCATTTGTATACGACTTAGAAAAAAAAAAAAAAAA138430.1282473169323516No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAA136030.12602385698409152No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGGTAAAAAAAAAAAA133640.12380966145228252No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGGTAAAAAAAAAAA133600.12377260378647818No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGGAAAAAAAAAAAA132040.12232735482010913No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACAAAAAAA131560.12188266283045711No Hit
CAAGACTGTAAATCTTGAGAAAAAAAAAAAAAAAAAAAAA131550.121873398414006No Hit
TTGATGATTCATAATAACTTTTCGAATCGCTAAAAAAAAA129510.11998345745798496No Hit
AAAGATTGGAAATCTTTTGAAAAAAAAAAAAAAAAAAAAA125290.11607387371562762No Hit
TTAAGGCAAAAGATTGGAAATCTTTTGAAAAAAAAAAAAA121980.11300735187031892No Hit
TTTGATGATTCATAATAACTTTTCGAATCGTAAAAAAAAA120320.11146945873943903No Hit
TTGATGATTCATAATAACTTTTCGAATCGTAAAAAAAAAA112680.10439144457081108No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA111020.10285355143993119No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)