FastQCFastQC Report
Mon 10 Jul 2023
SRR387900.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387900.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23255440
Total Bases930.2 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA9644254.147094185274499Illumina Single End Adapter 1 (95% over 22bp)
AGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATTCG7707593.3143169942172674No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCA3591251.5442623317382944No Hit
TAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATTC3148491.353872470269322No Hit
ATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATTCGTAT2113470.9088067136119548No Hit
AGTTTTGGTGGCAGGATACTGTAGGCACCATCAATTCGTA1825520.784986222578459No Hit
AAAAACCTAAAGGTAAACCTTTACTGTAGGCACCATCAAT1731620.7446085733058587No Hit
AGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCAT1691760.7274684976934429No Hit
AGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAA1649750.709403907214828No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCAC1613500.6938161565637976No Hit
ATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG1281660.5511226620524058No Hit
AGTTTTGGTGGCAGGATCTGTAGGCACCATCAATTCGTAT1273820.5477514078426381No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCAT1233390.5303662282889509No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA1153730.4961118774789898Illumina Single End Adapter 1 (95% over 22bp)
AAAAACCTAAAGGTAAACCTTTATCTGTAGGCACCATCAA1097080.4717519857719312No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACC1021910.4394283660081254No Hit
AGGGAACGTGAGCTGGGTTTACTGTAGGCACCATCAATTC1012770.4354981028094932No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCAC960830.4131635436697822No Hit
ACTGTAGAACTCTGAACCTGTAGGCACCATCAATTCGTAT870910.3744973219169364Illumina DpnII expression PCR Primer 2 (95% over 24bp)
ACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATTC776010.33368966572982495No Hit
AAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAA744660.32020894895989926No Hit
TGTACAACGGGGTATTGTAAGCCTGTAGGCACCATCAATT742780.31940053596061824No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATT720960.31001778508598415No Hit
TTTCCTTGTCTATGTTCCTTGGAACAGGACGTCCTGTAGG706540.30381708537873287No Hit
AGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAA702870.30223896000247685No Hit
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATC671970.2889517463440812No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCCTGTAGGCACCAT612110.2632115324414417No Hit
TAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAA593350.25514460272521183Illumina Single End Adapter 1 (95% over 22bp)
AGGGAACGTGAGCTGGGTTCTGTAGGCACCATCAATTCGT586100.25202705259500574No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGG557140.2395740523507618No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGC544970.23434086820116068No Hit
GTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA528450.22723715397343588Illumina Single End Adapter 1 (95% over 22bp)
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCAC500300.21513245932994604No Hit
TAGGGAACGTGAGCTGGGTTTACTGTAGGCACCATCAATT486780.2093187658457548No Hit
AACTTTTCGAATCGCACTGTAGGCACCATCAATTCGTATG481800.2071773314114891No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCCTGTAG469330.20181514518753463No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGACTGTAGGCACCA468730.2015571410388279No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCCTGTAGGCACCA458300.19707216892047622No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGCA455240.195756347762072No Hit
AAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAA433740.18651119910008152No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACC431720.18564258513276893No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCAC423780.18222833023155013No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACC423670.1821810294709539No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATC423620.18215952912522834No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACC418240.17984609192515816No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCA417750.17963538853704766No Hit
AGAATCCATGCTAGAACGCGGTGATTCTGTAGGCACCATC393900.1693797236259559No Hit
AGAGGGTGAGAATCCCGTGTGGCCTGTAGGCACCATCAAT390060.16772849707423296No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCA352790.15170213937040106No Hit
CAAGACTGTAAATCTTGAGCTGTAGGCACCATCAATTCGT348990.15006811309525858No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGG346140.14884259338890168No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTA331750.14265479388908575No Hit
GTGAATCATCGAATCTTTGAACGCACCTGTAGGCACCATC321580.13828162356850698No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA314260.13513397295428511Illumina Single End Adapter 1 (95% over 22bp)
AACTTTTCGAATCGCATCTGTAGGCACCATCAATTCGTAT309990.13329784342932235No Hit
AGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCAAT300570.12924717829462698No Hit
ACGGAGTCGAGCACCCGCAACGCGACTGTAGGCACCATCA295850.1272175456581342No Hit
TAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCA292450.12575552214879615No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACC280700.12070294090328973No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGC278770.11987302755828314No Hit
TTGGGCCAGCATCAGTTTTGGTGGCCTGTAGGCACCATCA266600.11463984340868202No Hit
AAAGATTGGAAATCTTTTGCTGTAGGCACCATCAATTCGT266040.11439903953655575No Hit
CAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATTC263500.11330682197369733No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACC254850.10958726216317558No Hit
AAAAAGCTCGTAGTTGAACTTTGCTGTAGGCACCATCAAT252400.10853374522262317No Hit
GAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATTC248140.10670191576680553No Hit
TTTTGGTGGCAGGATACTGTAGGCACCATCAATTCGTATG245700.10565269889539824No Hit
ACTTTGAAAAAATTAGAGTGTTCCTGTAGGCACCATCAAT239830.1031285583072176No Hit
ACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATT234460.10081942117629251No Hit
TTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCAC233070.10022171156512197No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)