FastQCFastQC Report
Mon 25 Sept 2023
SRR387902.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387902.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29610067
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAA14081264.755565058329656No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAA8924293.0139377935213725No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAA6254602.112322136927282No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAT4198091.4177914558585767No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAA3929471.3270723095628254No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAAAA3928191.3266400241512455No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAA3767521.2723780733086487No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA3240201.0942899926568894No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAA3032581.024171948006737No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAT2952740.9972081454594479No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA2899500.9792277741215513No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAA2799670.9455128892481062No Hit
TTTTATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAA2389630.8070329594323444No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAA2295650.7752937539790099No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGATGAAAAAAAAAAAAAAAAAA2048160.6917106942041029No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAAA2006700.6777086995446515No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA1924710.6500187925950995No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAATC1687730.5699852013168359No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAATC1672580.5648686982032158No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAATC1446300.488448742787377No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAAAAAAAATCGT1434650.4845142700960454No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA1432720.4838624647488977No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAA1408720.4757571132817768No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAT1365420.46113370834317935No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAA1268000.4282327358462241No Hit
TTGCTCGAATATATTAGCATGGAATAATAGGAAAAAAAAAAAAAAAAAAA1164060.3931298095340345No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA1073080.3624037730140901No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAA1054190.3560241859635103No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAT1043720.35248822638597876No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAA1038280.3506510133867647No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAA1014910.34275842739565565No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAAAAAAAAAAAAA969510.32742580420368517No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAATCG941680.3180269737316028No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAT933020.31510229274388335No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAT900270.3040418652210412No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACCAAAAAAAAAAAAAAAAAA887420.2997021249563535No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGGAAAAAAAAAAAAAAAAAA851830.28768256417656873No Hit
TTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAAAAAAAAAAAA836190.28240057680382824No Hit
TTTTCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAA814000.27490650392651933No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAAAAAAATCGTA804090.27155966921655394No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAA775480.26189741482179013No Hit
TTTTCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAA746820.25221827427813653No Hit
TGGTCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA688070.23237704933258005No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAATC681250.2300737786240065No Hit
TGGTCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAT679470.22947263172352836No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAC661100.22326866062140285No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAAAAAAAAAAAA652010.22019875875323078No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGGAAAAAAAAAAAAAAAAA642280.2169127141792688No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAAT611340.2064635652462387No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAC600750.20288707891137162No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAAA588100.19861488324224327No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAATCG566130.1911951094200496No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA552090.18645347881178384No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAATCG550710.1859874211024244No Hit
TTTTATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAAA548620.18528158007882928No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAT545540.1842413933072154No Hit
TTTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAAAAAAAAAAA535630.18089455859725004No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAATC506560.1710769516326998No Hit
TTGATGATTCATAATAACTTTTCGAATCGCTAAAAAAAAAAAAAAAAAAA495210.16724379583470717No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATGAAAAAAAAAAAAAAAA487290.16456902985055724No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTTGAAAAAAAAAAAAAAAAAAA469310.15849677070977247No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAAAAAAAAATCG462520.1562036316905328No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAA457970.1546669921415578No Hit
TTGTGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA452800.15292096434634883No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAATC451680.15254271461121652No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAT445860.15057716688043968No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACTAAAAAAAAAAAAAAAAAA435100.14694326763934712No Hit
TTGGTGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAA421260.14226918162664068No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAA417960.14115469579991158No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAA414470.13997604260740107No Hit
TTGATGATTCATAATAACTTTTCGAATCGCGAAAAAAAAAAAAAAAAAAA400160.1351432267951302No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTTGGAAAAAAAAAAAAAAAAAA398950.13473458199199617No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAAAAAAAAAAAA369800.12488995718922216No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAAAAAAAAAAAAA345900.11681837801988088No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAATC344270.11626788956607223No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGATGAAAAAAAAAAAAAAAAA326910.11040501867152142No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAAC326790.1103644919141858No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTTAAAAAAAAAAAAAAAAAAAA326640.1103138334675163No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAAAAAATCGTAT321200.10847662046830221No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGAAGAAAAAAAAAAAAAAAAAA310080.10472114095520282No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAAAAAAAAAAAA309310.10446109426229937No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAAAAAAAAAAAAT307300.10378227107692799No Hit
TTTATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAA305140.10305278944488712No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTTGATAAAAAAAAAAAAAAAAA298250.1007258781278678No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATGAAAAAAAAAAAAAAA296500.10013486291672356No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)