FastQCFastQC Report
Tue 26 Sept 2023
SRR387904.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387904.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161720098
Total Bases8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAA67321674.162851175121103No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAA45335952.803359048174705No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAAAA30179671.8661669373957464No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAA24065111.4880716928578661No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAA21006621.2989492499565514No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAA19650651.2151025285675996No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAA17880031.105615827662929No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAAA17825591.1022495175584175No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA15920280.9844342290715159No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAA14808840.9157080772978508No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA14402030.8905528860117312No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAT14288270.8835185098638761No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAT13159020.8136910725839407No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA11535240.713284257346913No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAA7710590.47678613204896775No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGATGAAAAAAAAAAAAAAAAAA7449030.4606125084094371No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA6779780.4192292784784239No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAATC6362110.39340255655793627No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAAAAAAAAAAAAA6220390.3846392672851336No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAT6112370.3779598253768063No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA6054490.37438080206951146No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAA6050920.3741600502863905No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAT5925590.3664102404884766No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAA5896100.3645867194564772No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAA5656690.3497827462360306No Hit
TTTTATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAA5638650.34866723862608595No Hit
TTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAAAAAAAAAAAA5612590.3470558124445361No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAG4701460.29071587626665923No Hit
TTGCTCGAATATATTAGCATGGAATAATAGGAAAAAAAAAAAAAAAAAAA4691570.29010432580865736No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAATC4534240.28037578854299233No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAA4363080.2697920699998586No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAA4340120.26837233304174724No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAAAAAAAATCGT4259240.2633710993670063No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA4132910.25555945433572513No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAATC4085390.25262104404611474No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAC3895160.24085812760266814No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAAAAAAAAAAAA3868720.23922320403243882No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAA3793490.2345713394262227No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAA3675660.22728529387856297No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAAA3663510.22653399579315123No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAAA3619910.22383797961834032No Hit
TTGATGATTCATAATAACTTTTCGAATCGCTAAAAAAAAAAAAAAAAAAA3619820.22383241444733729No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGGAAAAAAAAAAAAAAAAAA3484200.21544632009807466No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAC3480320.21520639939260983No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAN3435970.21246400679277352No Hit
TTTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAAAAAAAAAAA3375230.20870813471804847No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAG3365280.2080928741460446No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAT3185650.19698541117629054No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAT3121630.193026719536121No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACCAAAAAAAAAAAAAAAAAA3098800.1916150211583473No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATGAAAAAAAAAAAAAAAA3087420.19091133620262832No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAAA2837240.17544139751881674No Hit
TTGATGATTCATAATAACTTTTCGAATCGCGAAAAAAAAAAAAAAAAAAA2741770.16953798778924806No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAG2680840.16577036702018325No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAATCG2642540.16340207758221864No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAAAAAAAAAAAAA2632440.1627775417252097No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAAAAAAAAAAAA2601360.16085570267215643No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAAT2588700.1600728686177274No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAAAAAAAAAAA2520520.15585694240675022No Hit
TGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAAAAA2410860.14907609071570066No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAT2396400.14818195324121064No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAATC2385890.1475320649385211No Hit
AGATTTGAAATATTTATTATATAGATTTAAAAAAAAAAAAAAAAAAAATC2210700.1366991504049175No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGGAAAAAAAAAAAAAAAAA2194880.13572091701304806No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCTAAAAAAAAAAAAAAAAAA2148200.13283444831946614No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAATC2116420.1308693246030558No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2067880.12786784237541088No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATGAAAAAAAAAAAAAAA2052770.12693351199923214No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAATC2049130.12670843174977545No Hit
TTGATGATTCATAATAACTTTTCGAATCGTAAAAAAAAAAAAAAAAAAAA2011420.12437662509949754No Hit
TTGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA1996770.12347073893066772No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAAC1926370.11911753850161531No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACAAAAAAAAAAAAAAAAAA1908370.1180045043010053No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAC1857990.11488924524396465No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAAAAAAATCGTA1848790.11432036109698623No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAAT1802910.11148336059009809No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1787880.11055397703258873No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAGAAAAAAAAAAAAAAAAAA1781560.11016317835770789No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACAAAAAAAAAAAAAAAAA1742780.10776520800772703No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAAAAAAAAAAAA1710020.10573948576261684No Hit
TTTTCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAA1695450.10483854641245641No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAAAAAAAAAAAA1677790.10374653619119127No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAGTAAAAAAAAAAAAAAAAA1666020.10301873549445907No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAAAAAAAAAAA1654320.10229526326406257No Hit
TTGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAT1653870.10226743740904734No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCGAAAAAAAAAAAAAAAAAA1635490.1011309058197578No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)