FastQCFastQC Report
Tue 26 Sept 2023
SRR387905.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387905.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154043251
Total Bases7.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAA47281543.0693678361799828No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAA31958862.074667977501981No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAAAA29999561.9474764266043696No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAA24868291.6143706289345972No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAA24597971.596822310637939No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAA23377481.517591965129326No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAA19070621.2380042537533826No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA18371091.1925929815646386No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA14654560.9513276242137995No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAA13771620.894009955684459No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAA13642160.885605822484232No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAAA13382440.8687456226173778No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAT10659720.6919952630706294No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAT9732580.6318082705226729No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA8626950.5600342724524815No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAA7132590.4630251538900591No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGATGAAAAAAAAAAAAAAAAAA6204840.4027985620739723No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAATC5681190.3688048624733323No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAT5469970.3550931290069956No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAAAAAAAAAAA5457870.35430763532769116No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAA4946320.3210994294063555No Hit
TTTTATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAA4855370.31519524344497246No Hit
TTTTCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAA4573730.29691206659875025No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATGAAAAAAAAAAAAAAAA4168060.27057725495549295No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA3968000.25758999334544036No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAAA3886230.25228174391100067No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAAAAAAAAAAAA3829060.24857044856836993No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAT3755650.24380490385781328No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAAAAAAAAAAAAA3699080.24013255861498276No Hit
TTGCTCGAATATATTAGCATGGAATAATAGGAAAAAAAAAAAAAAAAAAA3593660.23328902608008448No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAA3222780.20921267105690985No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAATC3219820.20902051723122878No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAAAAAAAATCGT3155110.2048197489677753No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAT3146080.204233549965782No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAATC3122790.20272163692520356No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAAA3054890.19831378396447893No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAA3049760.19798076061118705No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA3000130.19475893818937903No Hit
TTGTGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA2994660.19440384311286704No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAT2859330.1856186481029279No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA2803830.18201576387140778No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACCAAAAAAAAAAAAAAAAAA2740180.17788380745093466No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGGAAAAAAAAAAAAAAAAAA2726500.17699574517548972No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATGAAAAAAAAAAAAAAA2703920.1755299230863415No Hit
TTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAAAAAAAAAAAA2651620.17213477272042252No Hit
TTTTCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAA2537060.16469790033190093No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACAAAAAAAAAAAAAAAAA2486100.16138973852220245No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAA2412950.1566410721882259No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAAAAAAAAAAA2407370.15627883626008388No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAC2389390.15511163160273733No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAAAAAAAAAAAA2276030.14775265941381618No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAGTAAAAAAAAAAAAAAAAA2198040.14268979560811787No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAAAAAAAAAAAA2185000.14184328010579314No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAA2151220.13965038948704087No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAATCG2137260.1387441504983558No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACAAAAAAAAAAAAAAAA2095480.13603192521560065No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAAAAAAAAAAA2067150.13419283133670037No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAA1983020.12873137817637983No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGGAAAAAAAAAAAAAAAAA1822350.11830119061821151No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAGTAAAAAAAAAAAAAAAA1791020.11626734624031014No Hit
TGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAAAAA1759310.11420883346586863No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAATC1749740.11358757937405516No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAA1729360.11226457431750775No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAC1727640.11215291736474713No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAAAAAAAAAA1659700.10774246773070247No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACAAAAAAAAAAAAAAAAAA1623920.10541974344594948No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATTAAAAAAAAAAAAAAAA1606860.10431226227496328No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAAC1600610.10390653206871102No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)