FastQCFastQC Report
Mon 25 Sept 2023
SRR387907.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387907.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31852735
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAA7372442.314539081180941No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAA4856111.5245504035995652No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAA3986701.251603669198265No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAAAT3247171.0194320833046204No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA3145630.9875541299671754No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAA2972650.9332479612818178No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAT2422120.7604119395084912No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACCAAAAAAAAAAAAAAAAAAAA2338280.7340908088426316No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAA2178920.6840605681113412No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAAAA1920590.6029592121367287No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAA1765180.5541690533010745No Hit
AGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAATCGTAT1634700.5132055379231956No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAA1448860.4548620393193865No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAA1357390.4261455099538548No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAAAAAAAAAAAA1303500.40922702555997154No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAATC1280230.40192153044314716No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAAAAAAAAAA1219730.3829278710289713No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCAAAAAAAAAAAAA1169440.36713958785642736No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA1090270.34228457933047196No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGGTAAAAAAAAAAAAAAAAAAAA1078090.3384607318649403No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAA1054780.33114267895676774No Hit
AGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAATCGTATG948910.2979053447058785No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGGAAAAAAAAAAAAAAAAAAA897590.281793698406118No Hit
ACTGCCAGCTGGGACTGAGGACTGCAAAAAAAAAAAAAAAAAAAATCGT882700.2771190605767448No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACTAAAAAAAAAAAAAAAAAAAA882180.27695580928921804No Hit
AGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAAATCGTATG880570.27645035818745234No Hit
ACTGCCAGCTGGGACTGAGGACTGCGATGAAAAAAAAAAAAAAAAAAAA872610.27395135770915746No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAAAAAAAATC867070.27221210360742965No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAAAAAAAAAAAA852430.26761595197398275No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAATC843600.2648438195338642No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA842610.26453301419799585No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAA841210.26409349150080835No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGTAAAAAAAAAAAAAAAAAAA839620.2635943192947168No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAA780280.24496483582963913No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAT765880.24044403094428154No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAAAAAAAAA761510.239072092239489No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAA744870.2338480510386314No Hit
TTTCCTTGTCTATGTTCCTTGGAACAGGACGTCAAAAAAAAAAAAAAAA740190.2323787894508902No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACCAAAAAAAAAAAAAAAAAAAT726060.22794274965713307No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAAAC652810.2049462942507135No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAA650970.20436863584869558No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACCAAAAAAAAAAAAAAAAA632720.19863914354607226No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAAAAAAAAAAA612610.19232571394575693No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAA601210.1887467434115155No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAA586270.18405640834295706No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAA581300.1824961027679413No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCAAAAAAAAAAAA577850.1814129932641577No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAAAAAAAAAAA574680.18041778829981162No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCAAAAAAAAAAAAAAAAAAAATCG559630.17569291930504555No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAATCG540220.169599251053324No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAA526490.165288789173049No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCAAAAAAAAAAAAAAAAAAA525980.16512867733335929No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAGAAAAAAAAAAAAAAAAAAAA507940.1594651134353141No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAAA499210.15672437547356607No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAAAAAAATCG485920.1525520492981215No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAAA484060.15196811200042948No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAA470670.14776439134661434No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCAAAAAAAAAAAAAA467320.1467126763212013No Hit
AGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAATCGTATGC447070.1403552944511672No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAA437300.13728805391436558No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGGTAAAAAAAAAAAAAAAAAAA426630.1339382630722291No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAC417150.13096206652270204No Hit
TTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAAAAAAAAAAA415420.13041894204689175No Hit
ACTGCCAGCTGGGACTGAGGACTGCAAAAAAAAAAAAAAAAAAATCGTA399640.12546489336002073No Hit
AGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAATCGTATGC398870.12522315587656757No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAAAAAAAAAA387930.12178859994283066No Hit
AGAATCCATGCTAGAACGCGGTGATTTAAAAAAAAAAAAAAAAAAAATC387540.12166616147718555No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA382560.12010271645433272No Hit
TTTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAAAAAAATCG379640.11918599768591301No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAA373320.11720186665289496No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAT368460.11567609500408678No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAAAC365050.1146055432916514No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCAAAAAAAAAAAAAAAAAAAATCG353020.1108287875436756No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAATCG348240.10932813147756384No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAAAAAAAAAAAA345200.10837373933509947No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAA336690.10570206922576664No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAAAA330070.10362375475763698No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAAAAAAAAAAAT329930.10357980248791823No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGGTAAAAAAAAAAAAAAAAAAAT328830.1032344632258423No Hit
AGCTGGGACTGAGGACTGCGACCAAAAAAAAAAAAAAAAAAAATCGTAT325510.10219216654394042No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAA323210.10147009354141802No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAT321440.1009144112742595No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAAAAAAAAT319300.10024256943712997No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)