FastQCFastQC Report
Tue 27 Jun 2023
SRR389612.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR389612.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22363895
Total Bases961.6 Mbp
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCA254681711.388074394017679No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCAC4207011.8811615776232182No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCA2682541.199495883878904No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCA2239401.0013461429683872No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCA1488800.6657158782045793No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCAC1467680.6562720849834074No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACC1321120.5907378835395176No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACC1305270.5836505671306362No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCA1304540.5833241481414574No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCAC1174380.5251231952215837No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGCACCA878700.39291009012517725No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCAC859880.38449474029456854No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACC738940.3304165039229526No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGC620460.27743825482993906No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGC434750.19439815828146215No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAA408120.18249057241594097Illumina Single End Adapter 1 (95% over 22bp)
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCA390180.17446871396954777No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCAC381030.17037729787230713No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCA373540.16702814961347295No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCAC364710.16307982129231066No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCA337360.15085028793061317No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA317810.14210851911082573No Hit
GGCGCCAGTGAAATACCACTACCTTTACTGTAGGCACCATCAA279460.12496034344643453No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCAC262630.11743482072331318No Hit
GGCGCCAGTGAAATACCACTACCTTTATCTGTAGGCACCATCA230360.10300531280441087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)