FastQCFastQC Report
Tue 27 Jun 2023
SRR389613.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR389613.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21130684
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA354858916.793535883646737No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA4547102.1518943731305624No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA3590791.699325019483515No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT1906080.9020436820691653No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1873880.8868051786681397No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA1762540.8341140305727918No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1428840.6761920248298635No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1323820.6264917879610522No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACCTGTAGGCACCATCAA1317390.6234488197353195No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1085750.5138262443373817No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA961350.45495451070112064No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT943230.44637930319718944No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT730220.3455732904812736No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATT708640.3353606537299029No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT532250.25188488929179953No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA469120.22200890420773886No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT419330.19844601338981738No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATT410160.19410635263865572No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATT377790.17878739751159972No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT311530.14743015417768776No Hit
TTTGCTCGAATATATTACCATGGAATAATAGAATCTGTAGGCACCATCAA276150.13068673025444894No Hit
TTTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA243360.11516901203955349No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATTCGT242600.11480934549965348No Hit
TTTGCTCGAATATATTATCATGGAATAATAGAATCTGTAGGCACCATCAA236930.11212604381382069No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)