FastQCFastQC Report
Tue 27 Jun 2023
SRR389614.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR389614.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18580228
Total Bases929 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA4102142.2077985264766395No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA2172521.1692644460552368No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1066470.5739811158399133No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT547140.2944743196908025No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG529090.284759691861693No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGC527470.2838877972864488No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATT485480.26128850517873087No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCC405360.21816739816109898No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT395290.21274765842485893No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT260480.14019203639481712No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT248050.1335021292526658No Hit
GGCGCCAGTGAAATACCACTACCTTTATCTGTAGGCACCATCAATTCGTA204810.1102300789850372No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGCACCATCAATTC199910.10759286699818754No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGCACTGTAGGCACCATCAATTCG197680.10639266644090696No Hit
GGCGCCAGTGAAATACCACTACCTTTACTGTAGGCACCATCAATTCGTAT186500.10037551745866627No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)