FastQCFastQC Report
Tue 27 Jun 2023
SRR389615.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR389615.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23023680
Total Bases920.9 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAG15647676.796337509902847No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGG8870473.8527594198668504No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAG2080610.903682643261199No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCA1616970.7023073635491807No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGG1134930.4929403118875871No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGC1048610.4554484773937094No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAG998230.4335666583274264No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGCA844840.36694394640648237No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGC631170.27413949464203813No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGC484110.21026612600592084No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA482670.20964068298378016Illumina Single End Adapter 1 (95% over 22bp)
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGG363950.15807638049173722No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGG359160.15599591377225536No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCAC325540.1413935565469986No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAG291660.12667827210941085No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGG283860.12329045573948215No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAG273110.11862134984502912No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGCACTGTAGGCAC272670.11843024225493058No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCAT263600.11449081988630835No Hit
GCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCA249720.10846224408956344No Hit

[OK]Adapter Content

Adapter graph