FastQCFastQC Report
Tue 27 Jun 2023
SRR389616.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR389616.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22479469
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA7679863.416388527682749No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1144700.509220213342228No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA695340.3093222531190572No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT524910.23350640533368472No Hit
GGCGCCAGTGAAATACCACTACCTTTACTGTAGGCACCATCAATTCGTAT474380.21102811636698357No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG468260.20830563213036749No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA458560.2039905835854041No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT449750.20007145186570022No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT424670.18891460469995977No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGC421090.18732204039161246No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT412260.18339401166459937No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA383240.17048445405894597No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATT367160.16333126018234684No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA338630.15063967925576888No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT332950.14811292917995528No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCC321620.14307277453929182No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA282480.12566133123518175No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGCACCATCAATTC252740.11243148136639704No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATT251220.11175530881089761No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA247220.10997590734905704No Hit
GGCGCCAGTGAAATACCACTACCTTTATCTGTAGGCACCATCAATTCGTA231870.10314745423924382No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)