FastQCFastQC Report
Mon 26 Jun 2023
SRR392976.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392976.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5486456
Total Bases395 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAA157680.2873986413087064TruSeq Adapter, Index 7 (96% over 28bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAA92530.16865167605463346Illumina Paired End PCR Primer 2 (100% over 24bp)
TTCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAA67270.12261102613417478RNA PCR Primer, Index 7 (96% over 28bp)
AAACTGGAGGAAGGTGGGGATGACGTCATCTCGTATGCCGTCTTCTGCTT64960.11840065791104495RNA PCR Primer, Index 41 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)