FastQCFastQC Report
Tue 27 Jun 2023
SRR392977.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392977.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24694844
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAA700770.2837717865316339TruSeq Adapter, Index 7 (96% over 28bp)
AAACTGGAGGAAGGTGGGGATGACGTCATCTCGTATGCCGTCTTCTGCTT314090.12718849327414258RNA PCR Primer, Index 41 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)