FastQCFastQC Report
Mon 26 Jun 2023
SRR392978.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392978.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3606003
Total Bases259.6 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACAGTCCGACGATCACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA67530.1872710588427131TruSeq Adapter, Index 13 (95% over 24bp)
AGTTCTACAGTCCGACGATCACTCGTATGCCGTCTTCTGCTTGAAAAAAA61860.1715472782468567TruSeq Adapter, Index 16 (95% over 24bp)
GTTCAAGTTCTACAGTCCGACGATCACTCGTATGCCGTCTTCTGCTTGAA43830.12154731984415985TruSeq Adapter, Index 13 (95% over 24bp)
CACGACCTCAGCTCAGACGAGAAGACCCATCTCGTATGCCGTCTTCTGCT36660.10166380893193933Illumina Paired End PCR Primer 2 (96% over 28bp)
TTCTACAGTCCGACGATCACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA36520.10127556743574533TruSeq Adapter, Index 16 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)