FastQCFastQC Report
Mon 26 Jun 2023
SRR392979.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392979.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15440923
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACAGTCCGACGATCACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA281200.18211346562637482TruSeq Adapter, Index 13 (95% over 24bp)
AGTTCTACAGTCCGACGATCACTCGTATGCCGTCTTCTGCTTGAAAAAAA249500.16158360481429768TruSeq Adapter, Index 16 (95% over 24bp)
CACGACCTCAGCTCAGACGAGAAGACCCATCTCGTATGCCGTCTTCTGCT184620.11956539126579414Illumina Paired End PCR Primer 2 (96% over 28bp)
CACGACCTCAGCTCAGACGAGAAGACCATCTCGTATGCCGTCTTCTGCTT167640.10856863932292131RNA PCR Primer, Index 16 (96% over 27bp)
CACGACCTCAGCTCAGACGAGAAGACCCGCATCTCGTATGCCGTCTTCTG155770.10088127503776814RNA PCR Primer, Index 18 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)