FastQCFastQC Report
Mon 26 Jun 2023
SRR392980.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392980.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3783159
Total Bases272.3 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAA497551.3151707342990342TruSeq Adapter, Index 7 (96% over 28bp)
AGCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAA282180.7458845901004955RNA PCR Primer, Index 16 (96% over 29bp)
TTCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAA268960.710940248612337RNA PCR Primer, Index 7 (96% over 28bp)
CTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAA241500.6383554061566009TruSeq Adapter, Index 7 (96% over 28bp)
TCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAA186210.49220770261043745RNA PCR Primer, Index 16 (96% over 29bp)
GTTCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAA167190.4419322582000915RNA PCR Primer, Index 16 (96% over 29bp)
TACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAA122750.32446429029284785RNA PCR Primer, Index 16 (96% over 29bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAA115740.30593480210585916Illumina Paired End PCR Primer 2 (100% over 24bp)
ACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA89100.23551746040808755Illumina PCR Primer Index 7 (96% over 28bp)
TTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAA65350.1727392372353369RNA PCR Primer, Index 7 (96% over 28bp)
AGTTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAA64930.17162905392028197RNA PCR Primer, Index 16 (96% over 29bp)
ATTCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAA59920.1583861529478407RNA PCR Primer, Index 16 (96% over 29bp)
AGTTCTACAGTCCGACGATCGACCGATCTCGTATGCCGTCTTCTGCTTGA54230.14334581232245328Illumina Paired End PCR Primer 2 (96% over 30bp)
AGTTTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAA46620.12323034797110034RNA PCR Primer, Index 16 (96% over 29bp)
ACTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAA45690.12077208491633579RNA PCR Primer, Index 7 (96% over 28bp)
AAGCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAA43940.11614632110360679RNA PCR Primer, Index 7 (96% over 28bp)
TCAAGCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGA43930.11611988816753406RNA PCR Primer, Index 16 (96% over 29bp)
AGCACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAA40780.10779351330462188RNA PCR Primer, Index 16 (96% over 29bp)
AGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA40050.10586390897131207RNA PCR Primer, Index 16 (96% over 29bp)
TCAAGTTCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTT38630.1021104320489834RNA PCR Primer, Index 16 (96% over 28bp)
GCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAA38280.10118527928643761RNA PCR Primer, Index 16 (96% over 29bp)
AGTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAA38250.10110598047821939RNA PCR Primer, Index 16 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)