FastQCFastQC Report
Mon 26 Jun 2023
SRR392981.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392981.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15690559
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAA1154100.7355378479504777TruSeq Adapter, Index 7 (96% over 28bp)
TTCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAA774740.49376188573013874RNA PCR Primer, Index 7 (96% over 28bp)
TCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAA560330.357112834539547RNA PCR Primer, Index 16 (96% over 29bp)
CTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAA530360.3380121766216232TruSeq Adapter, Index 7 (96% over 28bp)
GTTCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAA458960.2925071057060491RNA PCR Primer, Index 16 (96% over 29bp)
AGCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAA443450.2826221806374139RNA PCR Primer, Index 16 (96% over 29bp)
TCAAGTTCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTT178460.11373718425200785RNA PCR Primer, Index 16 (96% over 28bp)
TACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAA174070.11093932344921555RNA PCR Primer, Index 16 (96% over 29bp)
AGTTCTACAGTCCGACGATCGACCGATCTCGTATGCCGTCTTCTGCTTGA168100.1071344876877873Illumina Paired End PCR Primer 2 (96% over 30bp)
CAAGTTCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTG158310.1008950668997835TruSeq Adapter, Index 7 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)