FastQCFastQC Report
Mon 26 Jun 2023
SRR392982.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392982.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4095137
Total Bases294.8 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACAGTCCGACGATCACTCATCTCGTATGCCGTCTTCTGCTTGAAAAA183170.4472866231337315TruSeq Adapter, Index 23 (96% over 29bp)
AGTTCTACAGTCCGACGATCACTCATCTCGTATGCCGTCTTCTGCTTGAA146840.3585716424139168RNA PCR Primer, Index 45 (96% over 30bp)
TTCTACAGTCCGACGATCACTCATCTCGTATGCCGTCTTCTGCTTGAAAA120390.29398283866937786RNA PCR Primer, Index 45 (96% over 30bp)
CTACAGTCCGACGATCACTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA114310.2791359605307466RNA PCR Primer, Index 45 (96% over 30bp)
GTTCTACAGTCCGACGATCACTCATCTCGTATGCCGTCTTCTGCTTGAAA83460.20380270550167187TruSeq Adapter, Index 23 (96% over 29bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAA80500.19657462009207505Illumina Paired End PCR Primer 2 (100% over 24bp)
TACAGTCCGACGATCACTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAA61340.1497874185894147TruSeq Adapter, Index 23 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)