FastQCFastQC Report
Thu 29 Jun 2023
SRR392983.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392983.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20119718
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACAGTCCGACGATCACTCATCTCGTATGCCGTCTTCTGCTTGAAAAA729820.3627386825203017TruSeq Adapter, Index 23 (96% over 29bp)
CTACAGTCCGACGATCACTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA518570.2577421810782835RNA PCR Primer, Index 45 (96% over 30bp)
AGTTCTACAGTCCGACGATCACTCATCTCGTATGCCGTCTTCTGCTTGAA498080.24755814171948134RNA PCR Primer, Index 45 (96% over 30bp)
TTCTACAGTCCGACGATCACTCATCTCGTATGCCGTCTTCTGCTTGAAAA448640.22298523269560738RNA PCR Primer, Index 45 (96% over 30bp)
GTTCTACAGTCCGACGATCACTCATCTCGTATGCCGTCTTCTGCTTGAAA292640.14544935470765544TruSeq Adapter, Index 23 (96% over 29bp)
TACAGTCCGACGATCACTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAA268890.13364501430884868TruSeq Adapter, Index 23 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)