FastQCFastQC Report
Mon 26 Jun 2023
SRR392984.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392984.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2865982
Total Bases206.3 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGTCGTATGCCGTCTTCTGCTTGAAAAAA145190.506597738576167Illumina Single End Adapter 1 (95% over 22bp)
GTAGTTGGATACGAATAGGCCTTTGGCCCTGAATCGTATGCCGTCTTCTG81940.28590549417267797No Hit
AGTTCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAA69520.24256956254435652TruSeq Adapter, Index 7 (96% over 28bp)
TACCTGGTTGATTCTGCCAGTAGTCGTATGCCGTCTTCTGCTTGAAAAAA48830.17037790188493857Illumina Single End Adapter 1 (95% over 22bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAA44800.15631640394112734Illumina Paired End PCR Primer 2 (100% over 24bp)
GTTCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAA38790.1353462792159895RNA PCR Primer, Index 16 (96% over 29bp)
GTTGGATACGAATAGGCCTTTGGCCCTGAATCGTATGCCGTCTTCTGCTT32210.11238730738713641Illumina Single End Adapter 2 (95% over 21bp)
GGCCTTGGGGAGAAGCCTCTGGCGCAGAAGGGCAATCTCGTATGCCGTCT32200.11235241533268527No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)