FastQCFastQC Report
Thu 29 Jun 2023
SRR392985.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392985.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14948961
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGTCGTATGCCGTCTTCTGCTTGAAAAAA741750.4961883304130635Illumina Single End Adapter 1 (95% over 22bp)
GTAGTTGGATACGAATAGGCCTTTGGCCCTGAATCGTATGCCGTCTTCTG373410.24978993523362591No Hit
AGTTCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAA312840.20927206914246416TruSeq Adapter, Index 7 (96% over 28bp)
TACCTGGTTGATTCTGCCAGTAGTCGTATGCCGTCTTCTGCTTGAAAAAA257720.172399941373852Illumina Single End Adapter 1 (95% over 22bp)
GGCCTTGGGGAGAAGCCTCTGGCGCAGAAGGGCAATCTCGTATGCCGTCT186240.12458390920947617No Hit
GTTCTACAGTCCGACGATCGACCATCTCGTATGCCGTCTTCTGCTTGAAA177530.1187574173215115RNA PCR Primer, Index 16 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)