FastQCFastQC Report
Mon 26 Jun 2023
SRR392986.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392986.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3003575
Total Bases216.2 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT1797615.984901325919945RNA PCR Primer, Index 31 (95% over 21bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC690002.297262428938848RNA PCR Primer, Index 20 (95% over 24bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTC208360.6937066662227512No Hit
GCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA187930.6256877221311271RNA PCR Primer, Index 20 (96% over 30bp)
ACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGC178480.594225214952182RNA PCR Primer, Index 38 (100% over 25bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC168000.5593334609590239RNA PCR Primer, Index 31 (95% over 22bp)
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCT156830.5221444445369268RNA PCR Primer, Index 20 (96% over 25bp)
GTAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA154730.515152776274939RNA PCR Primer, Index 39 (96% over 28bp)
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTT154380.5139874982312744RNA PCR Primer, Index 3 (95% over 21bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT148810.4954429305078115TruSeq Adapter, Index 11 (95% over 24bp)
GTCCAGGGTTCAGGTCCCTGTTCGGATCTCGTATGCCGTCTTCTGCTTGA144250.4802610222817809Illumina PCR Primer Index 9 (96% over 28bp)
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTC141190.47007316281431294TruSeq Adapter, Index 3 (95% over 22bp)
GTCCTCGGTTCGTAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGC133810.4455024429221844Illumina PCR Primer Index 11 (95% over 24bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC133610.4448365697543761RNA PCR Primer, Index 31 (95% over 22bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGT128680.4284227961678999No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTT126080.4197664449863912TruSeq Adapter, Index 7 (95% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT112490.37452036323381305RNA PCR Primer, Index 41 (95% over 23bp)
TAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA111370.3707914734940862Illumina PCR Primer Index 11 (96% over 27bp)
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA108000.3595715106165153RNA PCR Primer, Index 31 (96% over 28bp)
GTCCAGGGTTCATGTCCCTGTTCGGATCTCGTATGCCGTCTTCTGCTTGA105340.3507153974846641Illumina PCR Primer Index 9 (96% over 28bp)
GTTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTC101920.33932896631514115RNA PCR Primer, Index 38 (100% over 22bp)
GTCCTCGGTTCGAAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGC100990.3362326560848322RNA PCR Primer, Index 39 (96% over 25bp)
CGCGAAAGGTCCTCGGTTCGTAACCGAGCAGAAACACATCTCGTATGCCG96340.32075110493328785No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC91290.3039378074461267RNA PCR Primer, Index 14 (95% over 22bp)
GTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTGCTT90770.302206537209825RNA PCR Primer, Index 24 (96% over 28bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCT89010.29634685333311134No Hit
CTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCT87500.29131951091615826RNA PCR Primer, Index 38 (96% over 27bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTC83950.27950026218755986No Hit
TACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTG82790.2756381978142713RNA PCR Primer, Index 38 (96% over 25bp)
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTATCTCGTATGCCGTCTTC76920.2560948203390959TruSeq Adapter, Index 10 (96% over 25bp)
ACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAA76590.25499612961221213RNA PCR Primer, Index 20 (96% over 30bp)
TTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTT73990.24633977843070343RNA PCR Primer, Index 38 (100% over 27bp)
GGCTTTGGTCTAGGGGTATGATTCTCGCTTATCTCGTATGCCGTCTTCTG73200.24370957941786037TruSeq Adapter, Index 10 (96% over 27bp)
CGGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGA72850.24254430137419575TruSeq Adapter, Index 3 (96% over 29bp)
CCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTT70830.23581898237933127RNA PCR Primer, Index 41 (95% over 21bp)
GTTACTTGGATAACTGTGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA69460.231257751179844RNA PCR Primer, Index 24 (96% over 29bp)
AGAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCT69310.23075834630398775RNA PCR Primer, Index 34 (95% over 22bp)
GCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGTC69150.23022564776974105No Hit
AGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTC68770.22896048875090516RNA PCR Primer, Index 34 (95% over 24bp)
CAGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTA67450.22456572584336998No Hit
GGTTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTT66260.22060378049491022RNA PCR Primer, Index 38 (95% over 22bp)
GTTTCTGTAGTGTAGTGGTCATCACGTTCGCCTATCTCGTATGCCGTCTT66100.22007108196066355Illumina PCR Primer Index 10 (95% over 21bp)
CGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAA64490.21471080295980624RNA PCR Primer, Index 41 (96% over 28bp)
GGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTTGA63600.21174766736305903RNA PCR Primer, Index 38 (96% over 29bp)
GTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA62100.2067536186044963RNA PCR Primer, Index 38 (100% over 27bp)
GAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTT60410.20112699033651565RNA PCR Primer, Index 24 (95% over 22bp)
CGCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCT59060.19663234645380923No Hit
GCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGC58330.19420190939130869TruSeq Adapter, Index 3 (96% over 25bp)
GAAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA56140.18691059820380712Illumina PCR Primer Index 11 (96% over 27bp)
GGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCT55770.18567873284336167RNA PCR Primer, Index 24 (95% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTC54980.18304853383051864No Hit
GGAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA54750.182282779687539RNA PCR Primer, Index 39 (96% over 28bp)
GGGGGTATAGCTCAGTGGTAGAGCATTTGACTATCTCGTATGCCGTCTTC53980.17971916799147683RNA PCR Primer, Index 37 (95% over 22bp)
GCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCT52640.1752578177671608No Hit
TCCCTGGTTGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT51430.17122928510192023No Hit
GTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTATG50600.1684659114555155No Hit
GTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA48570.16170729880226065RNA PCR Primer, Index 38 (100% over 27bp)
GGTTACTTGGATAACTGTGGCAATTCCAGAGCATCTCGTATGCCGTCTTC48380.1610747192928427TruSeq Adapter, Index 7 (96% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTATCTCGTATGCCGTCTTC48130.16024237783308226RNA PCR Primer, Index 27 (95% over 22bp)
GTTCTACAGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGAAA48040.1599427349075685RNA PCR Primer, Index 45 (100% over 27bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCG46760.15568114663359497No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCT45800.15248495542811483RNA PCR Primer, Index 31 (95% over 23bp)
TGGCGGCGGCGACCCTGGGCGCGCGCCGCGCCCTTCCCGCGGATCTCGTA45140.15028757397434722No Hit
GATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAA45110.150187692999176TruSeq Adapter, Index 18 (100% over 27bp)
CGCGAAAGGTCCTCGGTTCGAAACCGAGCAGAAACACATCTCGTATGCCG45050.14998793104883346No Hit
GCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTG44620.1485563037380455RNA PCR Primer, Index 34 (96% over 26bp)
GGTCCTCGGTTCGTAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTG44150.14699150179369583RNA PCR Primer, Index 39 (95% over 24bp)
ACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTCTGCT43960.1463589222842779RNA PCR Primer, Index 33 (100% over 25bp)
GAGCCGCGACTGGGGGAGCGGCCGCCCCGAGGTGCATCTCGTATGCCGTC43670.14539340619095578No Hit
GAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAA43160.14369542961304446RNA PCR Primer, Index 34 (96% over 30bp)
AACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAA42150.14033277011561224TruSeq Adapter, Index 18 (100% over 27bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTATCTCGTATGCCGTCTT42150.14033277011561224TruSeq Adapter, Index 10 (95% over 21bp)
GTCCTCGGTTCGGAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGC42150.14033277011561224Illumina PCR Primer Index 11 (95% over 24bp)
CGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTGCT41110.13687022964300877RNA PCR Primer, Index 34 (96% over 28bp)
GTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTT40980.1364374120839333RNA PCR Primer, Index 31 (96% over 27bp)
GCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTCT40850.13600459452485786No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGATCTCGTATGCCGTCTT40780.13577153891612495No Hit
TCCCATATGGTCTAGCGGTCAGGATTCCTGGTTATCTCGTATGCCGTCTT39940.13297487161132984Illumina PCR Primer Index 10 (95% over 21bp)
CTGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCG38690.12881316431252757No Hit
ACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA38300.12751471163530126TruSeq Adapter, Index 18 (96% over 28bp)
GAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGTCTTC38250.12734824334334918RNA PCR Primer, Index 20 (95% over 23bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGCATCTCGTATGCCG37990.1264826082251983No Hit
TGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTTG37420.12458486969694448RNA PCR Primer, Index 38 (96% over 29bp)
TCTACAGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGAAAAA36450.12135538483307393RNA PCR Primer, Index 45 (100% over 27bp)
TCAGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGT36300.12085597995721765No Hit
GTCCAGGGTTCAAGTCCCTGTTCGGATCTCGTATGCCGTCTTCTGCTTGA35950.11969070191355301Illumina PCR Primer Index 9 (96% over 28bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGATCTCGTATGCCGT34850.11602839949060703No Hit
GGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA34840.11599510583221662RNA PCR Primer, Index 34 (96% over 30bp)
CGCGAAAGGTCCTCGGTTCGGAACCGAGCAGAAACACATCTCGTATGCCG34470.11476324047177114No Hit
CTGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGT34380.11446359754625737No Hit
CGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGT33200.11053494585618803No Hit
GTTACTTGGATAACTGTGGCAATTCCAGAGCATCTCGTATGCCGTCTTCT32930.10963601707964675RNA PCR Primer, Index 26 (95% over 24bp)
GCAGCTAGCTGCGAGAACTAATGTGAATTGCAGGACAATCTCGTATGCCG32540.10833756440242046No Hit
GGTCCTCGGTTCGAAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTG31820.10594042099831034Illumina PCR Primer Index 9 (95% over 23bp)
TCCTCGTTAGTATAGTGGACAGTATCTCCGCCATCTCGTATGCCGTCTTC31120.10360986491098109RNA PCR Primer, Index 20 (100% over 21bp)
GGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGAA30480.10147907077399433RNA PCR Primer, Index 41 (96% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGCGCTATCTCGTATGCCGTCTT30450.10137918979882307No Hit
GATTCTAGGTTCGGCTCCTGGCTGGCTCGCATCTCGTATGCCGTCTTCTG30440.10134589614043264TruSeq Adapter, Index 18 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)