FastQCFastQC Report
Mon 26 Jun 2023
SRR392987.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392987.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13248077
Total Bases953.8 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT10140727.65448449612725RNA PCR Primer, Index 31 (95% over 21bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC6632055.0060472927504875RNA PCR Primer, Index 20 (95% over 24bp)
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTT2080131.5701373112490213RNA PCR Primer, Index 3 (95% over 21bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTC2038361.5386082070628062No Hit
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGT1437071.084738562434382No Hit
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTC1380871.0423173114105542TruSeq Adapter, Index 3 (95% over 22bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCT1134980.8567130157833472No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTT1070550.8080795424120799TruSeq Adapter, Index 7 (95% over 23bp)
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCT1050170.7926961777169622RNA PCR Primer, Index 20 (96% over 25bp)
ACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGC1047660.7908015631249727RNA PCR Primer, Index 38 (100% over 25bp)
GTCCTCGGTTCGAAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGC929440.7015659706687997RNA PCR Primer, Index 39 (96% over 25bp)
GCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGC841500.635186525561408TruSeq Adapter, Index 3 (96% over 25bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC826030.6235093591318952RNA PCR Primer, Index 31 (95% over 22bp)
CCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTT791050.5971055270889504RNA PCR Primer, Index 41 (95% over 21bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTC784120.5918745792313859No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTC669840.5056130033060647No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT631780.4768843055486468TruSeq Adapter, Index 11 (95% over 24bp)
GTTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTC629880.47545013514036794RNA PCR Primer, Index 38 (100% over 22bp)
CTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCT626900.4732007520789621RNA PCR Primer, Index 38 (96% over 27bp)
CGCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCT623590.4707022762624341No Hit
GCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGTC586130.4424264744234201No Hit
TACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTG563330.42521642952407357RNA PCR Primer, Index 38 (96% over 25bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC549320.41464130982934355RNA PCR Primer, Index 31 (95% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT485260.36628712227442517RNA PCR Primer, Index 41 (95% over 23bp)
CAGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTA467350.3527681791100701No Hit
CGCGAAAGGTCCTCGGTTCGAAACCGAGCAGAAACACATCTCGTATGCCG451940.3411363022723977No Hit
GTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTGCTT446690.33717346298636397RNA PCR Primer, Index 24 (96% over 28bp)
GGTTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTT445050.33593554747606014RNA PCR Primer, Index 38 (95% over 22bp)
CTGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCG441810.3334899095166793No Hit
GTTTCTGTAGTGTAGTGGTCATCACGTTCGCCTATCTCGTATGCCGTCTT438200.3307649857409494Illumina PCR Primer Index 10 (95% over 21bp)
TTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTT432080.3261454473732301RNA PCR Primer, Index 38 (100% over 27bp)
GAGCCGCGACTGGGGGAGCGGCCGCCCCGAGGTGCATCTCGTATGCCGTC431820.32594919247525506No Hit
CCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCT426860.3222052528831165TruSeq Adapter, Index 3 (95% over 23bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGCATCTCGTATGCCG415230.31342662033138846No Hit
AGAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCT397940.3003756696160507RNA PCR Primer, Index 34 (95% over 22bp)
AGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTC396750.29947742604454974RNA PCR Primer, Index 34 (95% over 24bp)
GAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGTCTTC382100.2884192173701889RNA PCR Primer, Index 20 (95% over 23bp)
GAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTT381730.2881399315538399RNA PCR Primer, Index 24 (95% over 22bp)
GCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCT363840.2746360849200982No Hit
GGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCT355900.2686427622665538RNA PCR Primer, Index 24 (95% over 24bp)
GCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTG348410.26298911155181237RNA PCR Primer, Index 34 (96% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTATCTCGTATGCCGTCTTC345650.26090579032715466RNA PCR Primer, Index 27 (95% over 22bp)
TGGCGGCGGCGACCCTGGGCGCGCGCCGCGCCCTTCCCGCGGATCTCGTA345260.2606114079801921No Hit
GGTTACTTGGATAACTGTGGCAATTCCAGAGCATCTCGTATGCCGTCTTC342840.25878472777596323TruSeq Adapter, Index 7 (96% over 25bp)
CTGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGT332600.2510553041018708No Hit
CGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTGCT325910.24600551461166778RNA PCR Primer, Index 34 (96% over 28bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCG322360.2433258804277783No Hit
GTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTATG320950.24226157501952925No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGGCTT316010.23853273195800417No Hit
CGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTG310400.2342981551209281RNA PCR Primer, Index 34 (95% over 24bp)
TCCCTGGTTGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT308490.2328564364473425No Hit
GGTCCTCGGTTCGAAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTG299960.22641776614070103Illumina PCR Primer Index 9 (95% over 23bp)
GCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTCT298180.22507417491610293No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCT294870.22257569909957498RNA PCR Primer, Index 31 (95% over 23bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGGC294140.22202467573218362No Hit
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCGCCCCCATCTCGTATGC291870.2203112195075557No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGATCTCGTATGCCGTCTT289970.21887704909927683No Hit
CGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGT276260.2085283773637487No Hit
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCGCCCCATCTCGTATGCC275780.20816606062902562No Hit
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGATCTCGTATGCCGT274880.20748671675141986No Hit
GAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTCTTCT260620.19672289042402152Illumina PCR Primer Index 3 (95% over 23bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGT259360.19577180899537344No Hit
CCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGC249910.18863869828051272RNA PCR Primer, Index 20 (96% over 27bp)
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCGCCCCCATCTCGTATGCC236220.17830512307559807No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTTGGCGCTATCTCGTATGCCGTCTT231830.17499143460594319No Hit
CAAAACCCATGCGGGTGCGCGGGCGGTGGAGAGGGGGATCTCGTATGCCG229900.1735346194017441No Hit
GTTACTTGGATAACTGTGGCAATTCCAGAGCATCTCGTATGCCGTCTTCT220930.16676382542160648RNA PCR Primer, Index 26 (95% over 24bp)
CGACCCACGTTCGGGCGCCCGAGGTCCGCGGCGACGCATCTCGTATGCCG216810.16365394011523335No Hit
CTTTCGAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCC216330.16329162338051023No Hit
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCATCTCGTATGCCGTCTT211980.16000812797208228RNA PCR Primer, Index 26 (95% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTTCTG208920.15769835878822264Illumina PCR Primer Index 7 (100% over 24bp)
TCAGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGT208130.1571020458289909No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCT203000.15322978572663792TruSeq Adapter, Index 18 (95% over 23bp)
TTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCT199350.15047466888968114RNA PCR Primer, Index 38 (95% over 24bp)
GACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTGC196540.14835360633848974RNA PCR Primer, Index 18 (96% over 25bp)
GTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTT192350.14519088317496948RNA PCR Primer, Index 31 (96% over 27bp)
CCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCT188820.14252634552169344RNA PCR Primer, Index 41 (96% over 26bp)
GACCGGGGCGCGACCCGCCCCGGGGACAGCGTCAGGTGGGGATCTCGTAT187330.14140165399099053No Hit
ATGAGGGGTGTAGAATAAGTGGGAGGCCCCCGGGCATCTCGTATGCCGTC184350.1391522709295847No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC183040.13816344817440296RNA PCR Primer, Index 14 (95% over 22bp)
AGCGGGCCTGGGCTCGAGCCGCGACTGGGGGAGCGGATCTCGTATGCCGT181190.13676701909265776No Hit
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCGCCCCATCTCGTATGCCG177330.1338533886842596No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTTCT164820.12441050878553922TruSeq Adapter, Index 7 (96% over 25bp)
AAAACCCATGCGGGTGCGCGGGCGGTGGAGAGGGGGATCTCGTATGCCGT163110.12311975541808823No Hit
GCAGCTAGCTGCGAGAACTAATGTGAATTGCAGGACAATCTCGTATGCCG161530.12192712949962474No Hit
GACCGGGGCGCGACCCGCCCCGGGGACAGCGTCAGGTGGGATCTCGTATG154490.11661315072368617No Hit
TGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTTG152510.11511859419295345RNA PCR Primer, Index 38 (96% over 29bp)
AGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTAT141100.10650602347797346No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTATCTCGTATGCCGTCTT138150.1042792852124878RNA PCR Primer, Index 10 (95% over 21bp)
CGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGTCTT136990.10340368643690703RNA PCR Primer, Index 20 (100% over 21bp)
AGCCGCGACTGGGGGAGCGGCCGCCCCGAGGTGCATCTCGTATGCCGTCT136840.10329046245730607No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)