FastQCFastQC Report
Mon 26 Jun 2023
SRR392988.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392988.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013291
Total Bases72.9 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCT289332.855349549142349RNA PCR Primer, Index 20 (96% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT275962.7234032474382976RNA PCR Primer, Index 31 (95% over 21bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC219502.166208917280426RNA PCR Primer, Index 20 (95% over 24bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTT77470.7645385185499526TruSeq Adapter, Index 7 (95% over 23bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC71520.705818960199982RNA PCR Primer, Index 31 (95% over 22bp)
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTG68640.6773967201919291RNA PCR Primer, Index 41 (95% over 24bp)
GAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTT66850.6597315085202573RNA PCR Primer, Index 24 (95% over 22bp)
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTC65980.6511456235178247TruSeq Adapter, Index 3 (95% over 22bp)
GCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCT62000.6118676668400291No Hit
GTTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTC60030.5924260651678541RNA PCR Primer, Index 38 (100% over 22bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC54240.5352855201516642RNA PCR Primer, Index 14 (95% over 22bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTC53040.5234429201483088No Hit
AGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTC51880.5119950734783986RNA PCR Primer, Index 34 (95% over 24bp)
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA47680.47054597346665467RNA PCR Primer, Index 31 (96% over 28bp)
ACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGC46660.4604797634638026RNA PCR Primer, Index 38 (100% over 25bp)
AGAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCT45700.45100568346111825RNA PCR Primer, Index 34 (95% over 22bp)
GATTCCCCGACGGGGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA42380.4182411567851684RNA PCR Primer, Index 24 (96% over 30bp)
GTCCTCGGTTCGTAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGC36920.36435732676990124Illumina PCR Primer Index 11 (95% over 24bp)
GCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA35280.34817244009864884RNA PCR Primer, Index 20 (96% over 30bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCT33570.33129673509386737No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT32820.3238951100917703TruSeq Adapter, Index 11 (95% over 24bp)
GGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCT30340.2994204034181691RNA PCR Primer, Index 24 (95% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT29140.2875778034148137RNA PCR Primer, Index 41 (95% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC26510.2616227717407931RNA PCR Primer, Index 31 (95% over 22bp)
TTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTT26380.26033982340709627RNA PCR Primer, Index 38 (100% over 27bp)
GCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGC25880.2554054067390315TruSeq Adapter, Index 3 (96% over 25bp)
TCAGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGT25620.25283951007163785No Hit
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTC24970.2464247684031537Illumina PCR Primer Index 3 (95% over 22bp)
GTTCTACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAAA23650.23339790839946276Illumina Paired End PCR Primer 2 (96% over 27bp)
TCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTATGC21970.21681826839476517No Hit
GTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTATG21470.21188385172670043No Hit
CTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCT21320.21040352672628101RNA PCR Primer, Index 38 (96% over 27bp)
CCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTT20450.20181764172384833RNA PCR Primer, Index 41 (95% over 21bp)
TACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTG19890.19629109505561582RNA PCR Primer, Index 38 (96% over 25bp)
GTCCTCGGTTCGAAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGC19680.19421864005502862RNA PCR Primer, Index 39 (96% over 25bp)
GCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTG19430.19175143172099623RNA PCR Primer, Index 34 (96% over 26bp)
GGTTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTT19270.19017241838721552RNA PCR Primer, Index 38 (95% over 22bp)
CAGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTA19040.18790258671990573No Hit
ATTCCCCGACGGGGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA19010.18760652171982184RNA PCR Primer, Index 26 (96% over 29bp)
AGCGGGCCTGGGCTCGAGCCGCGACTGGGGGAGCGGCATCTCGTATGCCG18870.18622488505276372No Hit
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTT18630.18385636505209263RNA PCR Primer, Index 3 (95% over 21bp)
TGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTTG16970.16747410171411767RNA PCR Primer, Index 38 (96% over 29bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTC16960.16737541338075637No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTATGCCGTCTTCTGCTTGAAA16840.16619115338042084Illumina PCR Primer Index 9 (96% over 27bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTTCTG16200.15987510004529795Illumina PCR Primer Index 7 (100% over 24bp)
ACCCGGGGACGCGTGCATTTATCAGATCATCTCGTATGCCGTCTTCTGCT15970.15760526837798816TruSeq Adapter, Index 7 (96% over 30bp)
ATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTG15940.15730920337790427Illumina PCR Primer Index 3 (96% over 28bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTC15920.15711182671118168No Hit
AATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTT15820.15612494337756871Illumina PCR Primer Index 3 (96% over 27bp)
GACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGTCTTCTGC15720.15513806004395578RNA PCR Primer, Index 26 (96% over 26bp)
CGCGCGGTGGCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCT15530.15326298171009117No Hit
TTCTACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAAAA15530.15326298171009117RNA PCR Primer, Index 18 (96% over 28bp)
GGAGGAGGGGGGGTGGAGAGGCCGTGGGGAGGATCTCGTATGCCGTCTTC15430.15227609837647824RNA PCR Primer, Index 26 (95% over 22bp)
GTTACTTGGATAACTGTGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA15360.15158528004294916RNA PCR Primer, Index 24 (96% over 29bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTTC15160.14961151337572326Illumina PCR Primer Index 7 (100% over 22bp)
GAGAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTC15150.14951282504236196No Hit
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGT15070.1487233183754716No Hit
AGATCCAAAACCCATGCGGGTGCGCGGGCGGTGGATCTCGTATGCCGTCT14890.1469469283749683RNA PCR Primer, Index 23 (95% over 21bp)
GCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTC14800.14605873337471664RNA PCR Primer, Index 26 (95% over 22bp)
CCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCT14570.14378890170740685TruSeq Adapter, Index 3 (95% over 23bp)
GATGCCTCCGCTGGCCGGGGATCTCGTATGCCGTCTTCTGCTTGAAAAAA14340.14151907004009706RNA PCR Primer, Index 23 (96% over 28bp)
TTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCT14320.14132169337337447RNA PCR Primer, Index 38 (95% over 24bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCG14090.1390518617060647No Hit
TCTACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAAAAA13820.13638727670530973Illumina Paired End PCR Primer 2 (96% over 27bp)
CGCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCT13800.13618990003858714No Hit
GCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGTC13730.1354990817050581No Hit
CGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGATCTCGTATGCCGTC13670.1349069517048903No Hit
GTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGCTTGA13670.1349069517048903TruSeq Adapter, Index 3 (96% over 28bp)
ACGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGATCTCGTATGCCGT13650.13470957503816772No Hit
GCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTCT13040.12868958670312872No Hit
GGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGA12930.12760401503615448RNA PCR Primer, Index 31 (96% over 28bp)
CGCGAAAGGTCCTCGGTTCGTAACCGAGCAGAAACACATCTCGTATGCCG12870.1270118850359867No Hit
AGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTAT12630.12464336503531562No Hit
GTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTT12610.12444598836859302RNA PCR Primer, Index 31 (96% over 27bp)
ACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGTCTTCTGCT12540.12375517003506396RNA PCR Primer, Index 26 (96% over 27bp)
GCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGTC12480.1231630400348962No Hit
CGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGT12350.12188009170119936No Hit
CGGAGGAGGGGGGGTGGAGAGGCCGTGGGGAGGATCTCGTATGCCGTCTT12280.1211892733676703RNA PCR Primer, Index 23 (95% over 21bp)
AGTTCTACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAA11910.11753780503330237TruSeq Adapter, Index 18 (96% over 28bp)
GGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTTGA11900.11743911669994109RNA PCR Primer, Index 38 (96% over 29bp)
CGGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGA11890.11734042836657979TruSeq Adapter, Index 3 (96% over 29bp)
ACGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCG11740.11586010336616037No Hit
CCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGC11550.11398502503229577RNA PCR Primer, Index 20 (96% over 27bp)
GCGCCGCCCAGGGCGGGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAA11520.11368896003221188Illumina PCR Primer Index 7 (100% over 28bp)
TGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTT11470.1131955183654054Illumina PCR Primer Index 3 (95% over 21bp)
GGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTCTGCTTGAAA11440.1128994533653215RNA PCR Primer, Index 18 (96% over 28bp)
ACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAA11380.11230732336515374RNA PCR Primer, Index 20 (96% over 30bp)
ACCCGGGGACGCGTGCATTTATCAGAATCTCGTATGCCGTCTTCTGCTTG11250.1110243750314569RNA PCR Primer, Index 40 (100% over 29bp)
ACCGCCGGTCCCGGACCCCCAGGCCATCTCGTATGCCGTCTTCTGCTTGA11180.11033355669792784RNA PCR Primer, Index 33 (96% over 31bp)
GTTTCTGTAGTGTAGTGGTCATCACGTTCGCCTATCTCGTATGCCGTCTT11150.11003749169784395Illumina PCR Primer Index 10 (95% over 21bp)
GGTCCTCGGTTCGTAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTG11010.10865585503078581RNA PCR Primer, Index 39 (95% over 24bp)
GATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTTGAA10660.10520176336314051RNA PCR Primer, Index 38 (96% over 29bp)
GTTACTTGGATAACTGTGGCAATTCCAGAGCATCTCGTATGCCGTCTTCT10620.10480701002969532RNA PCR Primer, Index 26 (95% over 24bp)
AAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGC10610.10470832169633401Illumina PCR Primer Index 3 (96% over 25bp)
TTGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCT10410.10273455502910812No Hit
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCGCCCCCATCTCGTATGCC10390.10253717836238554No Hit
TGGCGGCGGCGACCCTGGGCGCGCGCCGCGCCCTTCCCGCGGATCTCGTA10370.10233980169566295No Hit
GTGCGTGGGCGGTGGAGAGGGGGATCTCGTATGCCGTCTTCTGCTTGAAA10370.10233980169566295RNA PCR Primer, Index 23 (96% over 28bp)
GTCCTCGGTTCGGAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGC10230.10095816502860482Illumina PCR Primer Index 11 (95% over 24bp)
GTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTGCTT10210.10076078836188222RNA PCR Primer, Index 24 (96% over 28bp)
GCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGATCTCGTATGCCGTC10210.10076078836188222No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)