FastQCFastQC Report
Mon 26 Jun 2023
SRR392989.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392989.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6307324
Total Bases454.1 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC2798954.4376188697457115RNA PCR Primer, Index 20 (95% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT1593182.5259206598551147RNA PCR Primer, Index 31 (95% over 21bp)
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCT1552502.461424210964904RNA PCR Primer, Index 20 (96% over 25bp)
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTT502980.7974538805997599RNA PCR Primer, Index 3 (95% over 21bp)
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTG494120.7834067189191486RNA PCR Primer, Index 41 (95% over 24bp)
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTC414530.6572200825579912TruSeq Adapter, Index 3 (95% over 22bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTC390590.6192642077686195No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTT373760.5925809424091738TruSeq Adapter, Index 7 (95% over 23bp)
GAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTT314840.49916573177467966RNA PCR Primer, Index 24 (95% over 22bp)
CGGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGA291730.46252578748134704TruSeq Adapter, Index 3 (96% over 29bp)
CCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTT269170.426757845323944RNA PCR Primer, Index 41 (95% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC265780.4213831412497598RNA PCR Primer, Index 31 (95% over 22bp)
GTTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTC246830.39133870402091286RNA PCR Primer, Index 38 (100% over 22bp)
GCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGC241760.38330042978607093TruSeq Adapter, Index 3 (96% over 25bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCT240420.3811759154912606No Hit
GTCCTCGGTTCGTAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGC236890.3755792472370216Illumina PCR Primer Index 11 (95% over 24bp)
AGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTC236100.3743267350781409RNA PCR Primer, Index 34 (95% over 24bp)
GATTCCCCGACGGGGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA229070.3631809623225317RNA PCR Primer, Index 24 (96% over 30bp)
AGAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCT226500.359106334160097RNA PCR Primer, Index 34 (95% over 22bp)
GCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA214470.34003326925967337RNA PCR Primer, Index 20 (96% over 30bp)
TCAGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGT210280.3333901984423188No Hit
ACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGC200060.31718681329831794RNA PCR Primer, Index 38 (100% over 25bp)
CTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCT178210.28254454662547857RNA PCR Primer, Index 38 (96% over 27bp)
CGCGCGGTGGCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCT178150.2824494191197408No Hit
CAGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTA170170.2697974608566168No Hit
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTC165210.26193358704896086No Hit
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGT163550.25930172605688245No Hit
TTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTT159840.2534196752854301RNA PCR Primer, Index 38 (100% over 27bp)
CGCGAAAGGTCCTCGGTTCGTAACCGAGCAGAAACACATCTCGTATGCCG157900.250343885933242No Hit
CGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGATCTCGTATGCCGTC156540.24818766246985252No Hit
CGCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCT153830.24389107012736305No Hit
GCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCT152760.24219462960837274No Hit
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA151550.24027622490932765RNA PCR Primer, Index 31 (96% over 28bp)
CGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGT151020.23943593194197726No Hit
CGGAGGAGGGGGGGTGGAGAGGCCGTGGGGAGGATCTCGTATGCCGTCTT149510.23704188971424334RNA PCR Primer, Index 23 (95% over 21bp)
CCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCT148550.23551984962243894TruSeq Adapter, Index 3 (95% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC141980.22510338774415264RNA PCR Primer, Index 31 (95% over 22bp)
GTCCTCGGTTCGAAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGC135540.21489303546163158RNA PCR Primer, Index 39 (96% over 25bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC133690.21195993736805022RNA PCR Primer, Index 14 (95% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGGCTT131820.2089951301058896No Hit
GGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCT131050.20777432711558816RNA PCR Primer, Index 24 (95% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTC128340.2034777347730987No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT126380.20037023625233144RNA PCR Primer, Index 41 (95% over 23bp)
AGCGGGCCTGGGCTCGAGCCGCGACTGGGGGAGCGGCATCTCGTATGCCG110780.17563708476051018No Hit
TGGCGGCGGCGACCCTGGGCGCGCGCCGCGCCCTTCCCGCGGATCTCGTA109450.1735284250499895No Hit
TACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTG108920.17268813208263917RNA PCR Primer, Index 38 (96% over 25bp)
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTC108850.17257714999261178Illumina PCR Primer Index 3 (95% over 22bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT108370.17181612994670958TruSeq Adapter, Index 11 (95% over 24bp)
GTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTATG106490.16883546810025932No Hit
CGGGAGGGTCCTCGCCTGCCGCGCGATGCCTCCGCTGGCCGGGGATCTCG105170.1667426629740283No Hit
TGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTCTGCTTGAA104250.16528404121938242TruSeq Adapter, Index 18 (96% over 28bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTTCTG102120.16190701476569144Illumina PCR Primer Index 7 (100% over 24bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGGC101910.16157406849560924No Hit
CCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGC98640.15638961943290056RNA PCR Primer, Index 20 (96% over 27bp)
AATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTT97110.15396386803658732Illumina PCR Primer Index 3 (96% over 27bp)
ACCCGGGGACGCGTGCATTTATCAGATCATCTCGTATGCCGTCTTCTGCT96730.1533613938335814TruSeq Adapter, Index 7 (96% over 30bp)
TGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTTG95750.15180764457319776RNA PCR Primer, Index 38 (96% over 29bp)
GTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGCTTGA93570.14835134519805862TruSeq Adapter, Index 3 (96% over 28bp)
ATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTG93370.14803425351226607Illumina PCR Primer Index 3 (96% over 28bp)
ATTCCCCGACGGGGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA89990.14267540402237144RNA PCR Primer, Index 26 (96% over 29bp)
CGCGAAAGGTCCTCGGTTCGAAACCGAGCAGAAACACATCTCGTATGCCG89490.14188267480789No Hit
GGCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTTCTGCTTG87490.1387117579499642TruSeq Adapter, Index 18 (100% over 28bp)
TCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTATGC84810.13446272936034362No Hit
GCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTG84330.13370170931444142RNA PCR Primer, Index 34 (96% over 26bp)
GGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTCTGCTTGAAA84250.13357487264012438RNA PCR Primer, Index 18 (96% over 28bp)
GGTTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTT83880.13298825302140813RNA PCR Primer, Index 38 (95% over 22bp)
GACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGTCTTCTGC82380.13061006537796377RNA PCR Primer, Index 26 (96% over 26bp)
GCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTTCTGCTTGA77620.12306328325610036TruSeq Adapter, Index 18 (96% over 29bp)
GATGCCTCCGCTGGCCGGGGATCTCGTATGCCGTCTTCTGCTTGAAAAAA77110.12225469945732928RNA PCR Primer, Index 23 (96% over 28bp)
ACCCGGGGACGCGTGCATTTATCAGAATCTCGTATGCCGTCTTCTGCTTG76700.1216046615014545RNA PCR Primer, Index 40 (100% over 29bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTTC75700.1200192030724916Illumina PCR Primer Index 7 (100% over 22bp)
GTCCTCGGTTCGGAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGC75230.11927403761087904Illumina PCR Primer Index 11 (95% over 24bp)
GCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGTC71250.11296391306360669No Hit
TTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCT70690.11207605634338746RNA PCR Primer, Index 38 (95% over 24bp)
GGTCCTCGGTTCGTAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTG70180.11126747254461637RNA PCR Primer, Index 39 (95% over 24bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCG70020.11101379919598232No Hit
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCGCCCCATCTCGTATGCC69810.11068085292590012No Hit
CGCGGGGGTCGGGCGGGGGGGTATCTCGTATGCCGTCTTCTGCTTGAAAA69520.11022106998150086Illumina PCR Primer Index 3 (96% over 27bp)
AGATCCAAAACCCATGCGGGTGCGCGGGCGGTGGATCTCGTATGCCGTCT68300.10828681069816612RNA PCR Primer, Index 23 (95% over 21bp)
CGGTTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCT68270.10823924694529725No Hit
GAGAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTC68230.10817582860813872No Hit
GGAGGAGGGGGGGTGGAGAGGCCGTGGGGAGGATCTCGTATGCCGTCTTC67570.10712942604502321RNA PCR Primer, Index 26 (95% over 22bp)
CGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTG67490.10700258937070617RNA PCR Primer, Index 34 (95% over 24bp)
CGGAGGAGGGGGGGTGGAGAGGCATCTCGTATGCCGTCTTCTGCTTGAAA67380.10682818894352027RNA PCR Primer, Index 3 (96% over 29bp)
GCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGTC66560.10552811303177069No Hit
GCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAA66510.10544884011032254RNA PCR Primer, Index 26 (96% over 28bp)
AGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTAT65430.1037365450070426No Hit
GTTCTACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAAA65150.10329261664693298Illumina Paired End PCR Primer 2 (96% over 27bp)
ACCGCCGGTCCCGGACCCCCAGGCCATCTCGTATGCCGTCTTCTGCTTGA65000.10305479788258855RNA PCR Primer, Index 33 (96% over 31bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGCATCTCGTATGCCG64970.10300723412971968No Hit
CTGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGT63910.10132664819501899No Hit
GTTACTTGGATAACTGTGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA63520.10070831940772347RNA PCR Primer, Index 24 (96% over 29bp)
TGGCGGCGGCGACCCTGGGCGCGATCTCGTATGCCGTCTTCTGCTTGAAA63410.10053391898053754RNA PCR Primer, Index 33 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)