FastQCFastQC Report
Mon 26 Jun 2023
SRR392990.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392990.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405865
Total Bases173.2 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT1220325.072271303668327RNA PCR Primer, Index 31 (95% over 21bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC1006194.182237989247111RNA PCR Primer, Index 20 (95% over 24bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT425611.7690518794695465TruSeq Adapter, Index 11 (95% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT307221.2769627555993375RNA PCR Primer, Index 41 (95% over 23bp)
GCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA300961.2509430080241408RNA PCR Primer, Index 20 (96% over 30bp)
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTC282211.173008460574471TruSeq Adapter, Index 3 (95% over 22bp)
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTT246491.0245379520463533RNA PCR Primer, Index 3 (95% over 21bp)
GCTTTGGTGACTCTAGATAACCTCGTATGCCGTCTTCTGCTTGAAAAAAA221590.9210408730331918Illumina PCR Primer Index 12 (96% over 26bp)
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCT214140.8900748795131896RNA PCR Primer, Index 20 (96% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCATCTCGTATGCCGTCTTCTG193610.8047417456922978TruSeq Adapter, Index 18 (95% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCATCTCGTATGCCGTCTTC187720.7802599065201082TruSeq Adapter, Index 18 (95% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC166510.6921003464450416RNA PCR Primer, Index 31 (95% over 22bp)
GGTTACTTGGATAACTGTGGCAATTCCAGAGCATCTCGTATGCCGTCTTC158250.6577675804752137TruSeq Adapter, Index 7 (96% over 25bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTC140800.5852364949820543No Hit
GTGGCGCAGCGGAAGCGTGCTGGGCATCTCGTATGCCGTCTTCTGCTTGA130330.5417178436861586Illumina PCR Primer Index 3 (96% over 29bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGTATGCCGTCTTCTGCT93650.3892570863286178No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC91360.3797386802667647RNA PCR Primer, Index 31 (95% over 22bp)
GTTACTTGGATAACTGTGGCAATTCCAGAGCATCTCGTATGCCGTCTTCT82630.34345235497419846RNA PCR Primer, Index 26 (95% over 24bp)
AACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAA82170.34154036074343325TruSeq Adapter, Index 18 (100% over 27bp)
CGCTTTGGTGACTCTAGATAACCTCGTATGCCGTCTTCTGCTTGAAAAAA79960.33235447541736546Illumina PCR Primer Index 12 (96% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCATCTCGTATGCCGTCTTCTG78010.3242492824825998TruSeq Adapter, Index 3 (96% over 26bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGATCTCGTATGCCGTCTTCTG75750.3148555716966663Illumina PCR Primer Index 10 (96% over 26bp)
GTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA75420.3134839236615521RNA PCR Primer, Index 38 (100% over 27bp)
CCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTT73870.3070413344057127RNA PCR Primer, Index 41 (95% over 21bp)
GTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA73630.30604377219835693Illumina PCR Primer Index 3 (96% over 28bp)
ACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAA70610.29349111442246345RNA PCR Primer, Index 20 (96% over 30bp)
GCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGC70590.2934079842385171TruSeq Adapter, Index 3 (96% over 25bp)
ATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTG66150.2749530834024353Illumina PCR Primer Index 3 (96% over 28bp)
ACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGC65250.27121222512485116RNA PCR Primer, Index 38 (100% over 25bp)
CCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCT64610.26855205923856906RNA PCR Primer, Index 31 (95% over 23bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC63460.263772073661656RNA PCR Primer, Index 14 (95% over 22bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTC62980.26177694924694445No Hit
GTTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTC62510.25982338992420606RNA PCR Primer, Index 38 (100% over 22bp)
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTC61520.2557084458188635Illumina PCR Primer Index 3 (95% over 22bp)
GTGCGTGGGCGGTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA58850.2446105662620305RNA PCR Primer, Index 23 (96% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCATCTCGTATGCCGTCTTCT58270.24219979092758737TruSeq Adapter, Index 18 (96% over 29bp)
CGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAA55980.23268138486573436RNA PCR Primer, Index 41 (96% over 28bp)
GTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGCTTGA54530.22665444652962657TruSeq Adapter, Index 3 (96% over 28bp)
AATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTT52450.21800890739920986Illumina PCR Primer Index 3 (96% over 27bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTT50990.2119404039711289TruSeq Adapter, Index 7 (95% over 23bp)
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTATCTCGTATGCCGTCTTC48820.20292077901295377TruSeq Adapter, Index 10 (96% over 25bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCG45110.18750012989091241No Hit
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGT44470.18483996400463035No Hit
ACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTCTGCT43980.18280327449794564RNA PCR Primer, Index 33 (100% over 25bp)
GTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA43340.18014310861166358RNA PCR Primer, Index 38 (100% over 27bp)
GTTGCGCAGCGGAAGCGTGCTGGGCATCTCGTATGCCGTCTTCTGCTTGA43220.1796443275079857Illumina PCR Primer Index 3 (96% over 29bp)
GCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCT42870.1781895492889252No Hit
TACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTG41680.17324330334411947RNA PCR Primer, Index 38 (96% over 25bp)
ACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA40510.1683801875832601TruSeq Adapter, Index 18 (96% over 28bp)
TGGGCTCGAGCCGCGACTGGGGGAGCGGCCGCCATCTCGTATGCCGTCTT40490.16829705739931375No Hit
CTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCT40360.16775671120366273RNA PCR Primer, Index 38 (96% over 27bp)
ATTAAATCAGTTATGGTTCCTTTGATCGCTCCATCTCGTATGCCGTCTTC40350.16771514611168956TruSeq Adapter, Index 11 (95% over 23bp)
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA38560.1602749946484944RNA PCR Primer, Index 31 (96% over 28bp)
CCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCT38460.15985934372876284TruSeq Adapter, Index 3 (95% over 23bp)
ACCCGGGGACGCGTGCATTTATCAGATCATCTCGTATGCCGTCTTCTGCT38110.15840456550970233TruSeq Adapter, Index 7 (96% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTATCTCGTATGCCGTCTTC37750.15690822219866868RNA PCR Primer, Index 27 (95% over 22bp)
GGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTTGA37300.15503779305987658RNA PCR Primer, Index 38 (96% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGATCTCGTATGCCGTCTTCTGC36820.15304266864516503Illumina PCR Primer Index 9 (96% over 25bp)
AAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCT36180.15038250275888299Illumina PCR Primer Index 3 (96% over 26bp)
TTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTT35500.14755607650470828RNA PCR Primer, Index 38 (100% over 27bp)
AAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGC35200.14630912374551358Illumina PCR Primer Index 3 (96% over 25bp)
CGGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGA35120.1459766030097283TruSeq Adapter, Index 3 (96% over 29bp)
ATGGTTAGCACTCTGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA35110.14593503791775514Illumina PCR Primer Index 8 (96% over 29bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCATCTCGTATGCCGTCTTCT35010.14551938699802358TruSeq Adapter, Index 7 (95% over 24bp)
GGCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTTCTGCTTG34570.14369052295120466TruSeq Adapter, Index 18 (100% over 28bp)
TACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTCTGC34300.14256826546792942RNA PCR Primer, Index 21 (96% over 25bp)
GTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTATG33840.14065627123716418No Hit
GTTTCTGTAGTGTAGTGGTCATCACGTTCGCCTATCTCGTATGCCGTCTT33670.1399496646736205Illumina PCR Primer Index 10 (95% over 21bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGC33670.1399496646736205RNA PCR Primer, Index 20 (96% over 27bp)
TCCTCGTTAGTATAGTGGACAGTATCTCCGCATCTCGTATGCCGTCTTCT33190.13795454025890894TruSeq Adapter, Index 18 (100% over 24bp)
GGCTTTGGTCTAGGGGTATGATTCTCGCTTATCTCGTATGCCGTCTTCTG32870.13662445731576792TruSeq Adapter, Index 10 (96% over 27bp)
GTTACTTGGATAACTGTGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA32270.13413055179737848RNA PCR Primer, Index 24 (96% over 29bp)
ACCGCCGGTCCCGGACCCCCAGGCCATCTCGTATGCCGTCTTCTGCTTGA31130.12939213131243854RNA PCR Primer, Index 33 (96% over 31bp)
CGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTG30670.12748013708167333RNA PCR Primer, Index 34 (95% over 24bp)
GATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAA29500.12261702132081392TruSeq Adapter, Index 18 (100% over 27bp)
TCCTCGTTAGTATAGTGGACAGTATCTCCGCCATCTCGTATGCCGTCTTC28610.11891772813520293RNA PCR Primer, Index 20 (100% over 21bp)
AAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTG28220.11729668954824979Illumina PCR Primer Index 3 (95% over 24bp)
GGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTG27780.11546782550143088RNA PCR Primer, Index 41 (96% over 28bp)
GTTCTACAGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGAAA27720.11521843494959193RNA PCR Primer, Index 45 (100% over 27bp)
ACCGCCGGTCCCGGACCCCCAGGCATCTCGTATGCCGTCTTCTGCTTGAA27510.11434556801815562TruSeq Adapter, Index 3 (96% over 29bp)
GCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTCT27150.11284922470712197No Hit
GGGGAATTAGCTCAAGTGGTATCTCGTATGCCGTCTTCTGCTTGAAAAAA27050.11243357378739041TruSeq Adapter, Index 25 (96% over 28bp)
TCCCTGGTTGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT27010.11226731341949776No Hit
CTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA26710.11102036066030305RNA PCR Primer, Index 20 (96% over 30bp)
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCT26690.11093723047635674RNA PCR Primer, Index 30 (95% over 24bp)
CAGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTA26450.10993966826900096No Hit
TCTACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTCT25910.10769515330245048RNA PCR Primer, Index 21 (95% over 23bp)
GCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAA25850.10744576275061152RNA PCR Primer, Index 26 (96% over 28bp)
TGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTCTGCTTGAA25760.10707167692285312TruSeq Adapter, Index 18 (96% over 28bp)
TGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTT25580.10632350526733629Illumina PCR Primer Index 3 (95% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAATCTCGTATGCCGTCTTCT25390.1055337685198463RNA PCR Primer, Index 19 (95% over 24bp)
GGTTAGCACTCTGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA25230.10486872704827577Illumina PCR Primer Index 12 (96% over 28bp)
GTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA24900.10349707901316157RNA PCR Primer, Index 30 (100% over 28bp)
CACCGCCGGTCCCGGACCCCCAGGCCATCTCGTATGCCGTCTTCTGCTTG24630.10237482152988635RNA PCR Primer, Index 20 (96% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATCTCGTATGCCGTCTTC24600.10225012625396687RNA PCR Primer, Index 41 (95% over 22bp)
CCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCT24410.10146038950647689RNA PCR Primer, Index 41 (96% over 26bp)
GCGCCGCCCAGGGCGGGGATCATCTCGTATGCCGTCTTCTGCTTGAAAAA24290.100961608402799Illumina PCR Primer Index 7 (100% over 28bp)
ATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTTGA24270.10087847821885268RNA PCR Primer, Index 30 (96% over 29bp)
CTGGTTGATTCCGATAACGAACGAGACTCTGGCATCTCGTATGCCGTCTT24180.10050439239109427RNA PCR Primer, Index 34 (95% over 22bp)
GCGTTGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTG24080.1000887414713627TruSeq Adapter, Index 18 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)