FastQCFastQC Report
Mon 26 Jun 2023
SRR392991.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392991.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12960930
Total Bases933.1 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC10292357.941058241962576RNA PCR Primer, Index 20 (95% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT7193315.5499952549701295RNA PCR Primer, Index 31 (95% over 21bp)
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTT3798592.930800490396908RNA PCR Primer, Index 3 (95% over 21bp)
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTC2677002.0654382054374185TruSeq Adapter, Index 3 (95% over 22bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT2257691.7419197542151683TruSeq Adapter, Index 11 (95% over 24bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTC1511961.1665520915551584No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT1478581.1407977668269176RNA PCR Primer, Index 41 (95% over 23bp)
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCT1404611.0837262449531013RNA PCR Primer, Index 20 (96% over 25bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC1060660.8183517695103668RNA PCR Primer, Index 31 (95% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCATCTCGTATGCCGTCTTC1021460.7881070262704913TruSeq Adapter, Index 18 (95% over 22bp)
GCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGC959260.7401166428643624TruSeq Adapter, Index 3 (96% over 25bp)
CCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTT855540.6600915212102835RNA PCR Primer, Index 41 (95% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCATCTCGTATGCCGTCTTCTG853100.6582089402535157TruSeq Adapter, Index 18 (95% over 24bp)
GGTTACTTGGATAACTGTGGCAATTCCAGAGCATCTCGTATGCCGTCTTC765300.5904668877927741TruSeq Adapter, Index 7 (96% over 25bp)
GGCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTTCTGCTTG642120.49542741145889996TruSeq Adapter, Index 18 (100% over 28bp)
CCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCT617510.4764395764810087TruSeq Adapter, Index 3 (95% over 23bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTC580280.4477147858988514No Hit
CGGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGA513170.39593609409201347TruSeq Adapter, Index 3 (96% over 29bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGT471720.36395536431413483No Hit
CGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTG465660.3592797739051133RNA PCR Primer, Index 34 (95% over 24bp)
TGGGCTCGAGCCGCGACTGGGGGAGCGGCCGCCATCTCGTATGCCGTCTT452970.349488809830776No Hit
GTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGCTTGA446200.34426541922531795TruSeq Adapter, Index 3 (96% over 28bp)
GCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTTCTGCTTGA420010.32405853592296235TruSeq Adapter, Index 18 (96% over 29bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGATCTCGTATGCCGTCTTCTG417640.32222996343626575Illumina PCR Primer Index 10 (96% over 26bp)
CCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCT403420.31125852851608643RNA PCR Primer, Index 31 (95% over 23bp)
GTGGCGCAGCGGAAGCGTGCTGGGCATCTCGTATGCCGTCTTCTGCTTGA389960.3008734712709659Illumina PCR Primer Index 3 (96% over 29bp)
ATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTG385250.29723947278474616Illumina PCR Primer Index 3 (96% over 28bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGC381550.2943847393666967RNA PCR Primer, Index 20 (96% over 27bp)
GCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCT364200.28099835428476194No Hit
GTTACTTGGATAACTGTGGCAATTCCAGAGCATCTCGTATGCCGTCTTCT360550.27818219834533475RNA PCR Primer, Index 26 (95% over 24bp)
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTC355870.27457134634628844Illumina PCR Primer Index 3 (95% over 22bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC350660.2705515730738458RNA PCR Primer, Index 14 (95% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC347540.2681443384078149RNA PCR Primer, Index 31 (95% over 22bp)
AATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTT321000.2476674127551032Illumina PCR Primer Index 3 (96% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCATCTCGTATGCCGTCTTCTG320380.24718905202018682TruSeq Adapter, Index 3 (96% over 26bp)
CACCGCCGGTCCCGGACCCCCAGGCCATCTCGTATGCCGTCTTCTGCTTG319560.24655638137078126RNA PCR Primer, Index 20 (96% over 29bp)
CAGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTA311450.2402991143382458No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCG298870.23059302071687754No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTT297830.22979060916153393TruSeq Adapter, Index 7 (95% over 23bp)
GTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTATG290010.22375709150500772No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCATCTCGTATGCCGTCTTCT287670.2219516655054846TruSeq Adapter, Index 18 (96% over 29bp)
GTTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTC287330.22168933865085297RNA PCR Primer, Index 38 (100% over 22bp)
CCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCT275520.21257733820026803RNA PCR Primer, Index 41 (96% over 26bp)
TGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTCTGCTTGAA267580.20645123459504836TruSeq Adapter, Index 18 (96% over 28bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGCATCTCGTATGCCG253700.1957421265295006No Hit
ACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGC252540.19484712902546344RNA PCR Primer, Index 38 (100% over 25bp)
GGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTG251780.19426075135040463RNA PCR Primer, Index 41 (96% over 28bp)
GAGCCGCGACTGGGGGAGCGGCCGCCCCGAGGTGCATCTCGTATGCCGTC244870.1889293438048041No Hit
CGCGGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGC244240.18844326757416327TruSeq Adapter, Index 3 (96% over 26bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCATCTCGTATGCCGTCTTCT238720.18418431393426243TruSeq Adapter, Index 3 (95% over 24bp)
ACCGCCGGTCCCGGACCCCCAGGCCATCTCGTATGCCGTCTTCTGCTTGA228890.17659998163712018RNA PCR Primer, Index 33 (96% over 31bp)
ATTAAATCAGTTATGGTTCCTTTGATCGCTCCATCTCGTATGCCGTCTTC222510.17167749536491594TruSeq Adapter, Index 11 (95% over 23bp)
AAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCT217570.16786604047703366Illumina PCR Primer Index 3 (96% over 26bp)
CGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGCTT214370.1653970818452071TruSeq Adapter, Index 3 (96% over 27bp)
CGGTCCCGGACCCCCAGGCCTCCCGGCGCCCCCATCTCGTATGCCGTCTT213480.16471040272573034TruSeq Adapter, Index 18 (95% over 21bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGGC211840.16344506142691922No Hit
CGCCTACCCGCCGCCGTCCGCCCCCGGCATCTCGTATGCCGTCTTCTGCT206790.1595487360860679Illumina PCR Primer Index 3 (96% over 26bp)
AAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGC203320.15687145906968095Illumina PCR Primer Index 3 (96% over 25bp)
CTGGGCTCGAGCCGCGACTGGGGGAGCGGCCGCCATCTCGTATGCCGTCT203270.15683288159105865No Hit
CTGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGT201340.15534379091623826No Hit
TACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTG201010.15508917955733115RNA PCR Primer, Index 38 (96% over 25bp)
CTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCT195890.15113884574640862RNA PCR Primer, Index 38 (96% over 27bp)
CGGGGGCCCTGGTGGAGCGCCGAGAAGACGATCATCTCGTATGCCGTCTT195300.1506836314986656Illumina PCR Primer Index 7 (95% over 22bp)
ACCCGGGGACGCGTGCATTTATCAGATCATCTCGTATGCCGTCTTCTGCT194370.149966090396291TruSeq Adapter, Index 7 (96% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTATCTCGTATGCCGTCTTC191760.14795234601220744RNA PCR Primer, Index 27 (95% over 22bp)
GCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTCT191670.14788290655068734No Hit
GCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTC189770.1464169623630403RNA PCR Primer, Index 26 (95% over 22bp)
GTTTCTGTAGTGTAGTGGTCATCACGTTCGCCTATCTCGTATGCCGTCTT187410.1445961053720682Illumina PCR Primer Index 10 (95% over 21bp)
CACCGCCGGTCCCGGACCCCCAGGCATCTCGTATGCCGTCTTCTGCTTGA185150.1428524033383407RNA PCR Primer, Index 3 (96% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAATCTCGTATGCCGTCTTCT182820.14105469283454194RNA PCR Primer, Index 19 (95% over 24bp)
AGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTCTG179680.13863202717706213RNA PCR Primer, Index 26 (95% over 24bp)
GGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGA179550.1385317257326442RNA PCR Primer, Index 20 (96% over 30bp)
CGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTT179140.1382153904079414RNA PCR Primer, Index 20 (96% over 29bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCT178180.13747470281839344No Hit
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCATCTCGTATGCCGTCTT177710.1371120745193439RNA PCR Primer, Index 26 (95% over 21bp)
GCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGTC173280.133694109913409No Hit
AAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTG172170.13283768988799413Illumina PCR Primer Index 3 (95% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGATCTCGTATGCCGTCTTCTGC172120.13279911240937187Illumina PCR Primer Index 9 (96% over 25bp)
TCCCTGGTTGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT164190.12668072429987662No Hit
TTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTT163030.1257857267958395RNA PCR Primer, Index 38 (100% over 27bp)
GGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTTGA162430.125322797052372RNA PCR Primer, Index 38 (96% over 29bp)
TCCTCGTTAGTATAGTGGACAGTATCTCCGCATCTCGTATGCCGTCTTCT161530.12462840243717078TruSeq Adapter, Index 18 (100% over 24bp)
AGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTAT160240.1236331034887157No Hit
TGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTT157610.12160392811318324Illumina PCR Primer Index 3 (95% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGGCTT153930.11876462568658266No Hit
GCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTCTGC153240.11823225648159508RNA PCR Primer, Index 18 (96% over 25bp)
ACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTCTGCT152380.11756872384929168RNA PCR Primer, Index 33 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGCGCTATCTCGTATGCCGTCTT152190.11742212943052699No Hit
ACCGCCGGTCCCGGACCCCCAGGCATCTCGTATGCCGTCTTCTGCTTGAA148890.11487601584145582TruSeq Adapter, Index 3 (96% over 29bp)
AGCGGGCCTGGGCTCGAGCCGCGACTGGGGGAGCGGCATCTCGTATGCCG143860.11099512149205344No Hit
CAGATCCAAAACCCATGCGGGTGCGTGGGCGGTGGATCTCGTATGCCGTC142430.10989180560345593No Hit
TCCTCGTTAGTATAGTGGACAGTATCTCCGCCATCTCGTATGCCGTCTTC142340.1098223661419358RNA PCR Primer, Index 20 (100% over 21bp)
TCGCCTGCCGCGCGATGCCTCCGCTGGCCGGGGATCTCGTATGCCGTCTT140000.10801694014241262RNA PCR Primer, Index 23 (95% over 21bp)
CTGGTTGATTCCGATAACGAACGAGACTCTGGCATCTCGTATGCCGTCTT139860.10790892320227022RNA PCR Primer, Index 34 (95% over 22bp)
TACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTCTGC139100.1073225455272114RNA PCR Primer, Index 21 (96% over 25bp)
GGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGAA138670.1069907792110597RNA PCR Primer, Index 41 (96% over 28bp)
CGACCCACGTTCGGGCGCCCGAGGTCCGCGGCGACGCATCTCGTATGCCG136890.10561742097210616No Hit
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA136210.10509276726284303RNA PCR Primer, Index 31 (96% over 28bp)
AGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTC134610.10385828794692975RNA PCR Primer, Index 34 (95% over 24bp)
TCAGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGT134590.10384285695548082No Hit
CACCGCCGGTCCCGGACCCCCAGGATCTCGTATGCCGTCTTCTGCTTGAA133080.10267781710108766Illumina PCR Primer Index 1 (96% over 28bp)
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTATCTCGTATGCCGTCTTC132980.10260066214384307TruSeq Adapter, Index 10 (96% over 25bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATCTCGTATGCCGTCTTC132270.10205286194740655RNA PCR Primer, Index 41 (95% over 22bp)
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGATCTCGTATGCCGTCTTC132160.10196799149443751RNA PCR Primer, Index 46 (96% over 25bp)
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCT131940.10179825058849945RNA PCR Primer, Index 30 (95% over 24bp)
GGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTCTGCTTGAAA131200.10122730390488954RNA PCR Primer, Index 18 (96% over 28bp)
CGGCCGCCGGTGAAATACCACTACTCTTATCATCTCGTATGCCGTCTTCT130510.10069493469990194Illumina PCR Primer Index 7 (95% over 23bp)
AGCGGGCCTGGGCTCGAGCCGCGACTGGGGGAGCGGATCTCGTATGCCGT129780.1001317035120165No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)