FastQCFastQC Report
Mon 26 Jun 2023
SRR392992.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392992.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386837
Total Bases99.8 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCT519603.7466551584649093RNA PCR Primer, Index 20 (96% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT519323.744636175700533RNA PCR Primer, Index 31 (95% over 21bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC486123.505242505067286RNA PCR Primer, Index 20 (95% over 24bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT206881.4917398367652435TruSeq Adapter, Index 11 (95% over 24bp)
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTC199691.4398952436371397TruSeq Adapter, Index 3 (95% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC166001.1969683531662338RNA PCR Primer, Index 31 (95% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT162431.1712263229204296RNA PCR Primer, Index 41 (95% over 23bp)
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTG99980.7209210599371088RNA PCR Primer, Index 41 (95% over 24bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC93440.673763391083451RNA PCR Primer, Index 14 (95% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCATCTCGTATGCCGTCTTCTG86450.6233609285013307TruSeq Adapter, Index 18 (95% over 24bp)
GCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA78340.5648825348617033RNA PCR Primer, Index 20 (96% over 30bp)
GCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCT71170.513182154788198No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGATCTCGTATGCCGTCTTCTG66440.47907576737569013Illumina PCR Primer Index 10 (96% over 26bp)
ACCCGGGGACGCGTGCATTTATCAGATCATCTCGTATGCCGTCTTCTGCT64540.46537552718884767TruSeq Adapter, Index 7 (96% over 30bp)
CGCGCGGTGGCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCT61180.44114773401632634No Hit
ACCCGGGGACGCGTGCATTTATCAGATATCTCGTATGCCGTCTTCTGCTT58200.4196599888811735TruSeq Adapter, Index 25 (96% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCATCTCGTATGCCGTCTTC56190.40516657689404023TruSeq Adapter, Index 18 (95% over 22bp)
GTTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTC51800.37351181140970424RNA PCR Primer, Index 38 (100% over 22bp)
ACCCGGGGACGCGTGCATTTATCAGAATCTCGTATGCCGTCTTCTGCTTG49160.3544756882027232RNA PCR Primer, Index 40 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCATCTCGTATGCCGTCTTCTG48640.35072614878316627TruSeq Adapter, Index 3 (96% over 26bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTC46210.3332042626494678No Hit
GTTACTTGGATAACTGTGGCAATTCCAGAGCATCTCGTATGCCGTCTTCT44670.32209985744539554RNA PCR Primer, Index 26 (95% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC44260.3191434898261295RNA PCR Primer, Index 31 (95% over 22bp)
AGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTC41730.3009005384194393RNA PCR Primer, Index 34 (95% over 24bp)
CGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTG41570.2997468339826526TruSeq Adapter, Index 11 (96% over 25bp)
ACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGC41510.29931419481885757RNA PCR Primer, Index 38 (100% over 25bp)
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTT41280.29765574469097666RNA PCR Primer, Index 3 (95% over 21bp)
CCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCT41140.2966462533087883TruSeq Adapter, Index 3 (95% over 23bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCATCTCGTATGCCGTCTTCT37990.2739326972095495TruSeq Adapter, Index 18 (96% over 29bp)
CCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTT37730.2720579274997711RNA PCR Primer, Index 41 (95% over 21bp)
GTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA36890.26600097920664073RNA PCR Primer, Index 38 (100% over 27bp)
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTC35760.2578529416218345Illumina PCR Primer Index 3 (95% over 22bp)
TACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTG35150.253454443456585RNA PCR Primer, Index 38 (96% over 25bp)
GTTTCTGTAGTGTAGTGGTCATCACGTTCGCCTATCTCGTATGCCGTCTT34190.24653221683586463Illumina PCR Primer Index 10 (95% over 21bp)
TTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCT33260.23982630979704178RNA PCR Primer, Index 38 (95% over 24bp)
ATGGTTAGCACTCTGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA32730.2360046638501857Illumina PCR Primer Index 8 (96% over 29bp)
GCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTTCTGCTTGA29830.21509377093342621TruSeq Adapter, Index 18 (96% over 29bp)
GGTTACTTGGATAACTGTGGCAATTCCAGAGCATCTCGTATGCCGTCTTC29620.2135795338601436TruSeq Adapter, Index 7 (96% over 25bp)
GCGCGGTGGCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTT29510.2127863620598527TruSeq Adapter, Index 18 (95% over 22bp)
GTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA27900.20117721116468626RNA PCR Primer, Index 38 (100% over 27bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGATCTCGTATGCCGTCTTCTG26770.19302917357987998RNA PCR Primer, Index 23 (95% over 24bp)
GACGAGGTGGCCGAGTGGTTAAGGCTATGGAATCTCGTATGCCGTCTTCT26510.19115440387010152Illumina PCR Primer Index 8 (95% over 23bp)
GTGGCGCAGCGGAAGCGTGCTGGGCATCTCGTATGCCGTCTTCTGCTTGA26480.19093808428820402Illumina PCR Primer Index 3 (96% over 29bp)
CATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCTG26370.19014491248791315RNA PCR Primer, Index 20 (96% over 25bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTATCTCGTATGCCGTCTTCTGC25910.18682801223215129RNA PCR Primer, Index 38 (100% over 26bp)
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA25140.18127580963011516RNA PCR Primer, Index 31 (96% over 28bp)
GGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCT24910.17961735950223423RNA PCR Primer, Index 24 (95% over 24bp)
CGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTCT24700.17810312242895165RNA PCR Primer, Index 20 (95% over 23bp)
ACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAA24370.175723607028079RNA PCR Primer, Index 20 (96% over 30bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAATCTCGTATGCCGTCTTCT24290.17514675480968564RNA PCR Primer, Index 19 (95% over 24bp)
GGTTAGCACTCTGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA24190.17442568953669393Illumina PCR Primer Index 12 (96% over 28bp)
CCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCT23370.16851295429816193RNA PCR Primer, Index 31 (95% over 23bp)
GCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGC21880.15776908173058551TruSeq Adapter, Index 3 (96% over 25bp)
GTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGCTTGA21330.1538032227291311TruSeq Adapter, Index 3 (96% over 28bp)
GATTCCCCGACGGGGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA21180.15272162481964355RNA PCR Primer, Index 24 (96% over 30bp)
GTTCCATGGTGTAATGGTTAGCACTCTGGATCTCGTATGCCGTCTTCTGC20740.14954893761848004RNA PCR Primer, Index 30 (96% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTATCTCGTATGCCGTCTTC20550.1481789135997958RNA PCR Primer, Index 27 (95% over 22bp)
AATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTT20430.14731363527220573Illumina PCR Primer Index 3 (96% over 27bp)
ATTAAATCAGTTATGGTTCCTTTGATCGCTCCATCTCGTATGCCGTCTTC20000.1442130545983414TruSeq Adapter, Index 11 (95% over 23bp)
ATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTG19490.1405356217060837Illumina PCR Primer Index 3 (96% over 28bp)
CAGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTA19430.14010298254228867No Hit
GCGCGCGGTGGCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTC19230.13866085199630526No Hit
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTC19190.13837242588710857No Hit
CCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA19020.13714661492302266RNA PCR Primer, Index 41 (96% over 28bp)
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCT18750.13519973868594506RNA PCR Primer, Index 30 (95% over 24bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTT18700.1348392060494492TruSeq Adapter, Index 7 (95% over 23bp)
CGCGGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGC18630.134334460358355TruSeq Adapter, Index 3 (96% over 26bp)
GCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTC18240.13152230579368734RNA PCR Primer, Index 26 (95% over 22bp)
CGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTTCTGCTTGAA18170.13101756010259316RNA PCR Primer, Index 18 (96% over 29bp)
GCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTG17820.1284938316471222RNA PCR Primer, Index 34 (96% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATCTCGTATGCCGTCTTC17630.12712380762843795RNA PCR Primer, Index 41 (95% over 22bp)
TCAGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGT17590.12683538151924129No Hit
GTTCTACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAAA17470.12597010319165122Illumina Paired End PCR Primer 2 (96% over 27bp)
CTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCT17410.1255374640278562RNA PCR Primer, Index 38 (96% over 27bp)
TTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTT17070.1230858420996844RNA PCR Primer, Index 38 (100% over 27bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAATCTCGTATGCCGTCTTCT16950.12222056377209434RNA PCR Primer, Index 40 (100% over 24bp)
GCTCAGTCGGTAGAGCATGAGAATCTCGTATGCCGTCTTCTGCTTGAAAA16220.11695678727925488RNA PCR Primer, Index 26 (96% over 31bp)
GGCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTTCTGCTTG16130.11630782853356235TruSeq Adapter, Index 18 (100% over 28bp)
TGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTT16120.11623572200626317Illumina PCR Primer Index 3 (95% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCATCTCGTATGCCGTCTTCT15680.11306303480509966TruSeq Adapter, Index 18 (100% over 22bp)
AAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGC15290.11025088024043199Illumina PCR Primer Index 3 (96% over 25bp)
ACCCGGGGACGCGTGCATTTATCATCTCGTATGCCGTCTTCTGCTTGAAA15060.10859243011255108TruSeq Adapter, Index 7 (96% over 28bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGC15020.1083040040033544RNA PCR Primer, Index 20 (96% over 27bp)
GACGAGGTGGCCGAGTGGTTAAGGCTATGGATATCTCGTATGCCGTCTTC15010.10823189747605523RNA PCR Primer, Index 25 (95% over 22bp)
GTGCGTGGGCGGTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA15010.10823189747605523RNA PCR Primer, Index 23 (96% over 29bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGATCTCGTATGCCGTCTTCT14990.10808768442145689RNA PCR Primer, Index 33 (100% over 22bp)
GCGGAAGCGTGCTGGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA14680.10585238207518259Illumina PCR Primer Index 3 (100% over 27bp)
AAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCT14590.10520342332949006Illumina PCR Primer Index 3 (96% over 26bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCATCTCGTATGCCGTCTTCTGC14440.10412182542000249TruSeq Adapter, Index 18 (96% over 26bp)
GCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTCT14240.10267969487401908No Hit
CGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTT14160.1021028426556257TruSeq Adapter, Index 18 (95% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTATCTCGTATGCCGTCTT14040.10123756432803567RNA PCR Primer, Index 10 (95% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGATCTCGTATGCCGTCTTCTGC13900.10022807294584728Illumina PCR Primer Index 9 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)