FastQCFastQC Report
Mon 26 Jun 2023
SRR392993.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392993.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15334813
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC156627910.213877404308745RNA PCR Primer, Index 20 (95% over 24bp)
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCT6336064.131814323396053RNA PCR Primer, Index 20 (96% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT5244143.4197612973826286RNA PCR Primer, Index 31 (95% over 21bp)
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTT3338032.1767660290347197RNA PCR Primer, Index 3 (95% over 21bp)
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTC3169472.066846201515467TruSeq Adapter, Index 3 (95% over 22bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT2112961.377884425457291TruSeq Adapter, Index 11 (95% over 24bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTC2105791.37320878969962No Hit
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTG1834391.1962258685515108RNA PCR Primer, Index 41 (95% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT1735011.1314190789284486RNA PCR Primer, Index 41 (95% over 23bp)
GGCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTTCTGCTTG1697131.106717114841896TruSeq Adapter, Index 18 (100% over 28bp)
CCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTT1445520.9426394700737466RNA PCR Primer, Index 41 (95% over 21bp)
CGCGCGGTGGCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCT1440060.9390789440992857No Hit
GCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTTCTGCTTGA1313770.8567238478878093TruSeq Adapter, Index 18 (96% over 29bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC1169620.7627220494961366RNA PCR Primer, Index 31 (95% over 22bp)
GCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGC1143650.7457867272329959TruSeq Adapter, Index 3 (96% over 25bp)
CCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCT997640.650572002410463TruSeq Adapter, Index 3 (95% over 23bp)
CGGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGA977570.63748413495489TruSeq Adapter, Index 3 (96% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCATCTCGTATGCCGTCTTC962900.6279176668147176TruSeq Adapter, Index 18 (95% over 22bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC898470.5859021560941109RNA PCR Primer, Index 14 (95% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCATCTCGTATGCCGTCTTCTG876320.571457897791124TruSeq Adapter, Index 18 (95% over 24bp)
GCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCT820460.5350309782062553No Hit
CGCCTACCCGCCGCCGTCCGCCCCCGGCATCTCGTATGCCGTCTTCTGCT641370.4182444220219705Illumina PCR Primer Index 3 (96% over 26bp)
ACCCGGGGACGCGTGCATTTATCAGATCATCTCGTATGCCGTCTTCTGCT626630.40863230611289486TruSeq Adapter, Index 7 (96% over 30bp)
GCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTC562570.3668580764564915RNA PCR Primer, Index 26 (95% over 22bp)
CGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTG561550.3661929232524713RNA PCR Primer, Index 34 (95% over 24bp)
GTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGCTTGA540460.35243990259287805TruSeq Adapter, Index 3 (96% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCATCTCGTATGCCGTCTTCTG534520.3485663633459371TruSeq Adapter, Index 3 (96% over 26bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC532130.34700781809338005RNA PCR Primer, Index 31 (95% over 22bp)
ACCCGGGGACGCGTGCATTTATCAGATATCTCGTATGCCGTCTTCTGCTT521900.3403367227236485TruSeq Adapter, Index 25 (96% over 28bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGC491290.3203756054932003RNA PCR Primer, Index 20 (96% over 27bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTC466930.3044901819148365No Hit
CGCGGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGC454630.2964692168075346TruSeq Adapter, Index 3 (96% over 26bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGATCTCGTATGCCGTCTTCTG446780.2913501455805167Illumina PCR Primer Index 10 (96% over 26bp)
GCGCGGTGGCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTT443620.2892894748700229TruSeq Adapter, Index 18 (95% over 22bp)
AGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTC435860.284229093631595RNA PCR Primer, Index 34 (95% over 24bp)
ACCCGGGGACGCGTGCATTTATCAGAATCTCGTATGCCGTCTTCTGCTTG421980.2751777931690461RNA PCR Primer, Index 40 (100% over 29bp)
CAGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTA404030.26347240100026No Hit
TGGGCTCGAGCCGCGACTGGGGGAGCGGCCGCCATCTCGTATGCCGTCTT403730.26327676770495995No Hit
GTTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTC401980.26213557348237637RNA PCR Primer, Index 38 (100% over 22bp)
CCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCT389340.253892890640401RNA PCR Primer, Index 31 (95% over 23bp)
GGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCT383930.25036497021515686RNA PCR Primer, Index 24 (95% over 24bp)
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTC370570.24165276746446143Illumina PCR Primer Index 3 (95% over 22bp)
GTTACTTGGATAACTGTGGCAATTCCAGAGCATCTCGTATGCCGTCTTCT360210.23489689766676647RNA PCR Primer, Index 26 (95% over 24bp)
TCAGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGT356370.23239279148692585No Hit
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGT347440.2265694403968278No Hit
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGGC323830.21117310005671408No Hit
ATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTG302890.1975178960447708Illumina PCR Primer Index 3 (96% over 28bp)
TACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTG299580.19535940868662696RNA PCR Primer, Index 38 (96% over 25bp)
AATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTT298460.19462904438417344Illumina PCR Primer Index 3 (96% over 27bp)
TGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTCTGCTTGAA289270.18863614443814866TruSeq Adapter, Index 18 (96% over 28bp)
GGTTACTTGGATAACTGTGGCAATTCCAGAGCATCTCGTATGCCGTCTTC287670.18759276686321508TruSeq Adapter, Index 7 (96% over 25bp)
GTGGCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTTCTGCT286030.18652330484890817RNA PCR Primer, Index 18 (96% over 27bp)
CGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTTCTGCTTGAA274830.17921966182437307RNA PCR Primer, Index 18 (96% over 29bp)
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA274030.1786979730369063RNA PCR Primer, Index 31 (96% over 28bp)
ACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGC273720.1784958186317629RNA PCR Primer, Index 38 (100% over 25bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGCATCTCGTATGCCG272750.17786327097695942No Hit
CGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTT264990.17280288973853153TruSeq Adapter, Index 18 (95% over 21bp)
TTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCT257260.16776207182963365RNA PCR Primer, Index 38 (95% over 24bp)
GCCTACCCGCCGCCGTCCGCCCCCGGCATCTCGTATGCCGTCTTCTGCTT252650.16475584019185627Illumina PCR Primer Index 3 (96% over 27bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCATCTCGTATGCCGTCTTCT246810.1609475120433487TruSeq Adapter, Index 3 (95% over 24bp)
CGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTG246060.16045842880509856TruSeq Adapter, Index 11 (96% over 25bp)
GCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTG237380.15479810546108388RNA PCR Primer, Index 34 (96% over 26bp)
GGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTCTGCTTGAAA234310.15279612473918006RNA PCR Primer, Index 18 (96% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAATCTCGTATGCCGTCTTCT233840.15248963257654333RNA PCR Primer, Index 19 (95% over 24bp)
CTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCT232400.1515505927591031RNA PCR Primer, Index 38 (96% over 27bp)
GGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGA231710.15110063617991298RNA PCR Primer, Index 20 (96% over 30bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTT229540.14968555534390932TruSeq Adapter, Index 7 (95% over 23bp)
TGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTT222910.14536205951777828Illumina PCR Primer Index 3 (95% over 21bp)
AAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGC220780.14397306312114794Illumina PCR Primer Index 3 (96% over 25bp)
CACCGCCGGTCCCGGACCCCCAGGCCATCTCGTATGCCGTCTTCTGCTTG215450.14049731157465042RNA PCR Primer, Index 20 (96% over 29bp)
CGCCTACCCGCCGCCGTCCGCCCCCGGATCTCGTATGCCGTCTTCTGCTT214840.14009952387420702RNA PCR Primer, Index 30 (96% over 30bp)
ACCGCCGGTCCCGGACCCCCAGGCCATCTCGTATGCCGTCTTCTGCTTGA212050.13828013422791657RNA PCR Primer, Index 33 (96% over 31bp)
AAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCT211690.1380453742735565Illumina PCR Primer Index 3 (96% over 26bp)
AGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTCTG209350.13651943457021615RNA PCR Primer, Index 26 (95% over 24bp)
GTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTATG203350.1326067686642152No Hit
CCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCT203240.1325350364559385RNA PCR Primer, Index 41 (96% over 26bp)
CTGGGCTCGAGCCGCGACTGGGGGAGCGGCCGCCATCTCGTATGCCGTCT202540.13207855876690508No Hit
AGCGGGCCTGGGCTCGAGCCGCGACTGGGGGAGCGGCATCTCGTATGCCG200360.13065695682105807No Hit
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCATCTCGTATGCCGTCTT198960.1297440014429912RNA PCR Primer, Index 26 (95% over 21bp)
CGCGGTGGCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTTC197100.1285310750121309TruSeq Adapter, Index 18 (100% over 22bp)
CCCCTCCGGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCT193680.12630085544571037TruSeq Adapter, Index 3 (95% over 24bp)
GCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAA189820.12378370704618309RNA PCR Primer, Index 26 (96% over 28bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT188020.12260990727438281No Hit
GCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTCTGC186530.1216382619077259RNA PCR Primer, Index 18 (96% over 25bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCATCTCGTATGCCGTCTTCT185610.1210383198021391TruSeq Adapter, Index 18 (100% over 22bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTATCTCGTATGCCGTCTTCTGC183690.11978626671221879RNA PCR Primer, Index 38 (100% over 26bp)
AAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTG183450.11962976007597877Illumina PCR Primer Index 3 (95% over 24bp)
TTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTT180060.11741910383908823RNA PCR Primer, Index 38 (100% over 27bp)
CGCCCCCCTCCGGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTC178270.11625182517713127No Hit
CGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGCTT169440.11049368518546655TruSeq Adapter, Index 3 (96% over 27bp)
CTGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGT168220.1096981097845797No Hit
CGCGCCTACCCGCCGCCGTCCGCCCCCGGCATCTCGTATGCCGTCTTCTG167110.10897426659196953Illumina PCR Primer Index 3 (95% over 24bp)
CGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTCT165910.10819173341076933RNA PCR Primer, Index 20 (95% over 23bp)
GACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGTCTTCTGC161710.10545286727656868RNA PCR Primer, Index 26 (96% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTATCTCGTATGCCGTCTTC157290.10257053672581465RNA PCR Primer, Index 27 (95% over 22bp)
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCGCCCCATCTCGTATGCCG156250.10189234130210782No Hit
AGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTAT155270.101253272537461No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)