FastQCFastQC Report
Thu 29 Jun 2023
SRR392994.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392994.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3844975
Total Bases276.8 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT1562934.064863880779459TruSeq Adapter, Index 11 (95% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT1205523.1353129734263554RNA PCR Primer, Index 31 (95% over 21bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC940882.447038017152257RNA PCR Primer, Index 14 (95% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT705071.8337440425490414RNA PCR Primer, Index 41 (95% over 23bp)
GTTCTACAGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGAAA601261.5637552909966907RNA PCR Primer, Index 45 (100% over 27bp)
TCCTCGTTAGTATAGTGGACAGTATCTCCGCCATCTCGTATGCCGTCTTC408781.0631538566570655RNA PCR Primer, Index 20 (100% over 21bp)
ACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTCTGCT363590.9456238337050307RNA PCR Primer, Index 33 (100% over 25bp)
TCTACAGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGAAAAA363250.9447395626759602RNA PCR Primer, Index 45 (100% over 27bp)
CTACAGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA312210.8119948764296256RNA PCR Primer, Index 45 (96% over 30bp)
AGTTCTACAGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGAA301410.7839062672709186RNA PCR Primer, Index 45 (96% over 30bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGATCTCGTATGCCGTCTTCTG289560.7530868211106705Illumina PCR Primer Index 10 (96% over 26bp)
TTCTACAGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGAAAA284180.7390945324742033RNA PCR Primer, Index 45 (96% over 30bp)
TCCTCGTTAGTATAGTGGACAGTATCTCCGCATCTCGTATGCCGTCTTCT279000.7256224032666012TruSeq Adapter, Index 18 (100% over 24bp)
TACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTCTGC233080.6061937983992094RNA PCR Primer, Index 21 (96% over 25bp)
CTACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTCTG210630.5478059025091191RNA PCR Primer, Index 21 (95% over 24bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCATCTCGTATGCCGTCTTCTGCT179580.46705115117783597RNA PCR Primer, Index 24 (96% over 27bp)
GTACTCTGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA174350.4534489821130176RNA PCR Primer, Index 21 (96% over 28bp)
TCTACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTCT171010.444762319650973RNA PCR Primer, Index 21 (95% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCATCTCGTATGCCGTCTTC161660.4204448663515367TruSeq Adapter, Index 18 (95% over 22bp)
TACAGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAA156620.40733684874414006RNA PCR Primer, Index 45 (100% over 27bp)
ACAGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA150040.3902236035344833RNA PCR Primer, Index 45 (96% over 30bp)
GTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA141970.36923517057978267RNA PCR Primer, Index 38 (100% over 27bp)
TCTACAGTCCGACGATCTTTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA136450.35487877034311016TruSeq Adapter, Index 13 (95% over 24bp)
CTACAGTCCGACGATCTTTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA135160.35152374202693126TruSeq Adapter, Index 16 (95% over 24bp)
GTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA130520.3394560432772645Illumina PCR Primer Index 3 (96% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCATCTCGTATGCCGTCTTCTG125250.32574984232667314TruSeq Adapter, Index 3 (96% over 26bp)
GTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA115290.29984590276919876RNA PCR Primer, Index 38 (100% over 27bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTATCTCGTATGCCGTCTTCTGC111190.28918263447746734RNA PCR Primer, Index 38 (100% over 26bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC106560.2771409436992438RNA PCR Primer, Index 20 (95% over 24bp)
GTGCGTGGGCGGTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA106080.27589256106996796RNA PCR Primer, Index 23 (96% over 29bp)
TGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA105160.27349982769718917Illumina PCR Primer Index 3 (96% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCATCTCGTATGCCGTCTTCTG102840.2674659783223558TruSeq Adapter, Index 18 (95% over 24bp)
CAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTCTGCTT99590.2590133876033004RNA PCR Primer, Index 21 (96% over 27bp)
TTCTACAGTCCGACGATCTTTCGTATGCCGTCTTCTGCTTGAAAAAAAAA98190.25537227160124576TruSeq Adapter, Index 16 (95% over 24bp)
TCCTCGTTAGTATAGTGGACAGTATCTCCGCCTGTATCTCGTATGCCGTC96370.25063882079857475No Hit
TACAGTCCGACGATCTTTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA90550.23550218141860482TruSeq Adapter, Index 13 (95% over 24bp)
AGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA89520.23282336035995033RNA PCR Primer, Index 45 (100% over 27bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAA87830.2284280131860415Illumina Paired End PCR Primer 2 (100% over 24bp)
TTCTACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTC84830.22062562175306732RNA PCR Primer, Index 33 (100% over 21bp)
GTTCTACAGTCCGACGATCTTTCGATCTCGTATGCCGTCTTCTGCTTGAA82380.2142536687494717RNA PCR Primer, Index 21 (100% over 29bp)
GTTCAGAGTTCTACAGTCGACGATCTTTCATCTCGTATGCCGTCTTCTGC82350.21417564483514195RNA PCR Primer, Index 45 (96% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATCTCGTATGCCGTCTTC81390.2116788795765902RNA PCR Primer, Index 41 (95% over 22bp)
AGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTCTGCTTG79610.2070494606596922RNA PCR Primer, Index 33 (100% over 27bp)
ACAGTCCGACGATCTTTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA78390.20387648814361603TruSeq Adapter, Index 16 (95% over 24bp)
GTGGCGCAGCGGAAGCGTGCTGGGCATCTCGTATGCCGTCTTCTGCTTGA74590.19399345899518203Illumina PCR Primer Index 3 (96% over 29bp)
GTTCTACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTT74310.1932652357947711RNA PCR Primer, Index 21 (95% over 21bp)
GCGTTGGTGGTGTAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC62570.1627318773203987RNA PCR Primer, Index 20 (95% over 22bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCG62550.16267986137751222No Hit
GCGTTGGTGGTATAGTGGTTAGCATAGCTGCCATCTCGTATGCCGTCTTC58920.15323896774361342RNA PCR Primer, Index 14 (95% over 22bp)
GCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA58230.15144441771402936RNA PCR Primer, Index 20 (96% over 30bp)
GTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTGCTT57950.15071619451361842RNA PCR Primer, Index 24 (96% over 28bp)
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA57480.1494938198557858RNA PCR Primer, Index 31 (96% over 28bp)
AGTTCTACAGTCCGACGATCTTTCGATCTCGTATGCCGTCTTCTGCTTGA56030.14572266399651493RNA PCR Primer, Index 21 (96% over 30bp)
GGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA54540.14184747625147107Illumina PCR Primer Index 3 (96% over 28bp)
ACAGTCCGACGATCTTTCTACTCTGCGATCTCGTATGCCGTCTTCTGCTT53400.1388825675069409RNA PCR Primer, Index 21 (96% over 27bp)
GTTCTACAGTCCGACGATCTTTCGTATGCCGTCTTCTGCTTGAAAAAAAA53170.1382843841637462TruSeq Adapter, Index 13 (95% over 24bp)
GATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAA53000.137842248649211TruSeq Adapter, Index 18 (100% over 27bp)
GTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA52600.1368019297914811TruSeq Adapter, Index 18 (96% over 28bp)
GGTTAGCACTCTGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA50920.13243259058901552Illumina PCR Primer Index 12 (96% over 28bp)
TAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA50030.13011788113056652Illumina PCR Primer Index 11 (96% over 27bp)
ACAGTCCGACGATCTTTCGCACTCTGGAATCTCGTATGCCGTCTTCTGCT49840.1296237296731448Illumina PCR Primer Index 8 (96% over 27bp)
GTATAGTGGACAGTATCTCCGCATCTCGTATGCCGTCTTCTGCTTGAAAA49360.12837534704386894TruSeq Adapter, Index 18 (96% over 30bp)
AGTTCTACAGTCCGACGATCTTTCGTATGCCGTCTTCTGCTTGAAAAAAA49090.12767313181490128TruSeq Adapter, Index 16 (95% over 24bp)
TCTACAGTCCGACGATCTTTCGATCTCGTATGCCGTCTTCTGCTTGAAAA47880.12452616727026834RNA PCR Primer, Index 21 (100% over 29bp)
GTACTCTGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC47600.12379794406985742RNA PCR Primer, Index 21 (96% over 28bp)
CTACAGTCCGACGATCTTTCGATCTCGTATGCCGTCTTCTGCTTGAAAAA46720.12150924258285165RNA PCR Primer, Index 21 (96% over 30bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC46520.12098908315398671RNA PCR Primer, Index 31 (95% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC45400.11807619035234299RNA PCR Primer, Index 31 (95% over 22bp)
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTATCTCGTATGCCGTCTTC45320.117868126580797TruSeq Adapter, Index 10 (96% over 25bp)
CAGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA42820.11136613371998517RNA PCR Primer, Index 45 (96% over 30bp)
AGTCCGACGATCTTTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA42700.1110540380626662TruSeq Adapter, Index 16 (95% over 24bp)
ACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA42370.11019577500503905TruSeq Adapter, Index 18 (96% over 28bp)
AGTTCTACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCT41940.1090774322329794No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTT39650.10312160677247575TruSeq Adapter, Index 7 (95% over 23bp)
AACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAA39610.10301757488670278TruSeq Adapter, Index 18 (100% over 27bp)
GCGTTGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTCT39100.10169116834309716RNA PCR Primer, Index 14 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)